| Retrovirus vector production and transduction: modulation by the cell cycle. | |
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MedLine Citation:
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PMID: 14573819 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study, the cell cycle modulation of retrovirus vector production and transduction was analysed. Retrovirus vector expression was found to be similar in all phases of the cell cycle and, in contrast to some other virus promoters shown previously to be upregulated by G(2)/M arrest, Moloney murine leukaemia virus LTR-driven expression was upregulated neither by G(2)/M growth arrest nor by G(1)/S growth arrest. In contrast, cultures enriched for S phase cells produced more infectious virions, apparently by modulation of stages consequent to provirus expression. In terms of retrovirus transduction, limitations appear to be slow progression through the cell cycle and short half-life of the virus. Synchronization of cells prior to mitosis can increase transduction efficiency. Cell cycle modulation can be used to modify retrovirus vector production and transduction and can allow short transduction periods. |
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Authors:
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Alla Dolnikov; Simon Wotherspoon; Michelle Millington; Geoff Symonds |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of general virology Volume: 84 ISSN: 0022-1317 ISO Abbreviation: J. Gen. Virol. Publication Date: 2003 Nov |
Date Detail:
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Created Date: 2003-10-23 Completed Date: 2003-11-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0077340 Medline TA: J Gen Virol Country: England |
Other Details:
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Languages: eng Pagination: 3131-41 Citation Subset: IM |
Affiliation:
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Department of Clinical Pharmacology and Toxicology, St Vincent's Hospital, Darlinghurst, NSW 2010, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle* Cell Line G2 Phase Genetic Vectors* Humans Mitosis Retroviridae / genetics*, growth & development* Transduction, Genetic* Virus Cultivation* Virus Integration |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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