Document Detail

Retrovirally mediated delivery of angiotensin II type 1 receptor antisense in vitro and in vivo.
MedLine Citation:
PMID:  10565039     Owner:  NLM     Status:  MEDLINE    
In spite of excellent drugs that are available for the control of hypertension, the pharmacological approach has major disadvantages including compliance, side effects, and inability to cure the disease. In the present chapter we provide evidence that a gene therapy concept based on the inhibition of the RAS at a genetic level, with the use of an antisense to the AT1R, is an exciting and viable approach for long-term control of hypertension without the disadvantages inherent in pharmaceutical therapy. A retrovirus-based vector has been used to deliver AT1R-AS in Ang II target tissues both in vitro and in vivo. The transduction efficiency is high and leads to the attenuation of Ang II action in vitro and prevention of hypertension in the SH rat, a model for primary human hypertension. These studies have unveiled a new avenue in which a similar approach could be attempted in the reversal of hypertension in adult animals.
H Wang; D Lu; P Y Reaves; M J Katovich; M K Raizada
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Methods in enzymology     Volume:  314     ISSN:  0076-6879     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2000  
Date Detail:
Created Date:  1999-12-06     Completed Date:  1999-12-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  581-90     Citation Subset:  IM    
Department of Physiology, University of Florida College of Medicine, Gainesville 32610, USA.
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MeSH Terms
DNA, Antisense / therapeutic use*
Drug Delivery Systems
Genetic Vectors*
Hypertension / drug therapy*
Rats, Inbred SHR
Rats, Inbred WKY
Receptor, Angiotensin, Type 1
Receptor, Angiotensin, Type 2
Receptors, Angiotensin / genetics*
Grant Support
Reg. No./Substance:
0/DNA, Antisense; 0/Receptor, Angiotensin, Type 1; 0/Receptor, Angiotensin, Type 2; 0/Receptors, Angiotensin

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