Document Detail


Retroviral transduction in fetal thymic organ culture.
MedLine Citation:
PMID:  15492404     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
T-cell development requires cytokines and intimate contact with stromal cells provided exclusively by the thymus. Consequently, an in vitro model of thymocyte differentiation, fetal thymic organ culture (FTOC), has been developed. FTOC recapitulates the normal development of T-cells derived from both mouse and human progenitor populations, providing a more rapid means to study T-cell development compared with alternative in vivo approaches. Furthermore, FTOC is easily amenable to genetic manipulation using retroviral gene transfer. In this chapter, we outline the basic FTOC technique and describe several applications, including retroviral transduction of mouse thymocyte subsets and human CD34+ stem/progenitor cells.
Authors:
Bronwyn M Owens; Robert G Hawley; Lisa M Spain
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  Methods in molecular medicine     Volume:  105     ISSN:  1543-1894     ISO Abbreviation:  Methods Mol. Med.     Publication Date:  2005  
Date Detail:
Created Date:  2004-10-19     Completed Date:  2005-03-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  101123138     Medline TA:  Methods Mol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  311-22     Citation Subset:  IM    
Affiliation:
Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation / genetics,  physiology*
Hematopoietic Stem Cells / physiology
Humans
Lymphopoiesis / genetics,  physiology*
Mice
Organ Culture Techniques / methods
Retroviridae*
T-Lymphocytes / physiology*
Thymus Gland / cytology,  physiology*
Transduction, Genetic / methods*
Grant Support
ID/Acronym/Agency:
AI 36453/AI/NIAID NIH HHS; AI 49807/AI/NIAID NIH HHS; HL 65519/HL/NHLBI NIH HHS; HL 66305/HL/NHLBI NIH HHS

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