Document Detail


Retroviral transduction of alveolar bone cells with a temperature-sensitive SV40 large T antigen.
MedLine Citation:
PMID:  11456413     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have transduced adult human alveolar bone (AB) cells with a gene construct encoding a temperature-sensitive mutation of the SV40 large T antigen (tsT). Such cells divided rapidly, for more than 50 passages thus far, at a permissive low temperature (34.5 degrees C), comparable to the non-transduced parental cells at 37 degrees C. However, the tsT-transduced AB cells failed to grow at a non-permissive high temperature (39 degrees C) at which the T antigen is inactivated. Nevertheless, the cells formed mineralised nodules in vitro at both the low and high temperatures. Flow cytometry analysis showed that the transduced cells cultured at 34.5 degrees C, like the parental cells at 37 degrees C, were smaller and less granular than the transduced cells incubated at 39 degrees C. Moreover, the transduced cells grown at 34.5 degrees C were also found to express bone sialoprotein, osteopontin and type I collagen at levels similar to those of the parental cells at 37 degrees C, although osteonectin and fibronectin were down-regulated. When the transduced cells were incubated at 39 degrees C, the expression of all antigens was up-regulated, particularly osteonectin. Thus, we have obtained long-term cultures of tsT-transduced AB cells whose growth is temperature-dependent and which express certain features characteristic of bone-derived cells.
Authors:
V Salih; J C Knowles; M J O'Hare; I Olsen
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Cell and tissue research     Volume:  304     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-07-17     Completed Date:  2002-01-22     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  371-6     Citation Subset:  IM    
Affiliation:
Department of Biomaterials, Eastman Dental Institute, University College London, UK.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Polyomavirus Transforming / genetics*,  metabolism
Biological Markers / analysis
Cell Division
Cell Line
Cell Size
Cell Transformation, Viral*
Cytoplasmic Granules / metabolism
Extracellular Matrix Proteins / immunology,  metabolism
Flow Cytometry
Hot Temperature
Humans
Kinetics
Mutation
Osteoblasts / cytology*,  metabolism
Retroviridae / genetics*
Sialoglycoproteins / immunology,  metabolism
Staining and Labeling
Tooth Socket / cytology*
Transduction, Genetic
Chemical
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming; 0/Biological Markers; 0/Extracellular Matrix Proteins; 0/Sialoglycoproteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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