Document Detail

Retrospective assessment of liver cell proliferation via PCNA: a comparison with tritiated thymidine.
MedLine Citation:
PMID:  7910515     Owner:  NLM     Status:  MEDLINE    
Cell proliferation (S phase response) in archival liver tissues of partially hepatectomized rats was determined via proliferating cell nuclear antigen (PCNA) immunohistochemistry. These results were compared with the S phase response assessed previously in the same tissues via tritiated thymidine (Tdr) autoradiography. The effect of prolonged tissue fixation on PCNA immunohistochemistry was compared in two studies: study A, the liver was fixed for a maximum of 7 days and then embedded in paraffin and stored for approximately 18 months, while in study B, the liver was fixed in formalin for 7 years and then embedded in paraffin and stored for approximately 18 months until sectioning and immunostaining. PCNA immunostaining was successful in the liver sections of both studies, irrespective of the length of formalin fixation. Furthermore, the S phase labeling indices (LI) determined via PCNA and Tdr were comparable, although not identical, in the two studies. Therefore, use of PCNA immunohistochemistry should allow retrospective staining of rodent tissues for the assessment of cell proliferative activity in formalin-fixed organs from previously conducted toxicity and carcinogenicity studies.
D R Dietrich; R Candrian; D S Marsman; J A Popp; W K Kaufmann; J A Swenberg
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Cancer letters     Volume:  79     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-06-21     Completed Date:  1994-06-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  45-51     Citation Subset:  IM    
Institute of Toxicology ETH, Schwerzenbach, Switzerland.
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MeSH Terms
Antigens, Neoplasm / analysis
Autoantigens / analysis
Cell Division*
Immunohistochemistry / methods
Liver / cytology*,  physiology
Liver Regeneration*
Mitotic Index
Nuclear Proteins / analysis*
Proliferating Cell Nuclear Antigen
Rats, Inbred F344
Retrospective Studies
Thymidine / metabolism*
Reg. No./Substance:
0/Antigens, Neoplasm; 0/Autoantigens; 0/Nuclear Proteins; 0/Proliferating Cell Nuclear Antigen; 10028-17-8/Tritium; 50-89-5/Thymidine

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