Document Detail


Retrieval of a metabolite from cells with polyelectrolyte microcapsules.
MedLine Citation:
PMID:  20882557     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
To monitor cellular processes in individual cells, it is important to measure the concentrations of intracellular metabolites and to retrieve them for analysis. The use of functionalized polyelectrolyte microcapsules as intracellular sensors for in vivo reporting is persented. Capsules loaded with streptavidin-rhodamine, which was introduced into fibroblasts by electroporation, autonomously escaped from an endocytic compartment and efficiently recruited biotin-fluorescein from the cytosol. This work demonstrates the utility of polyelectrolyte microcapsules for intracellular capture of metabolites and eventually for drug delivery on an organismic level.
Authors:
Deborah Studer; Raghavendra Palankar; Matthieu Bédard; Mathias Winterhalter; Sebastian Springer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Small (Weinheim an der Bergstrasse, Germany)     Volume:  6     ISSN:  1613-6829     ISO Abbreviation:  Small     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101235338     Medline TA:  Small     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2412-9     Citation Subset:  IM    
Affiliation:
Biophysics, Jacobs University Bremen, 28759 Bremen, Germany.
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