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Retrieval of HDEL proteins is required for growth of yeast cells.
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MedLine Citation:
PMID:  7929564     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ERD2 gene of Saccharomyces cerevisiae encodes the receptor which retrieves HDEL-containing containing ER proteins from the Golgi apparatus. Viable erd2 mutants have been isolated that show no obvious HDEL-dependent retention of the luminal ER protein BiP, suggesting that retrieval of HDEL proteins is not essential for growth. However, cells that lack Erd2p completely have a defective Golgi apparatus and cannot grow. This observation led to the suggestion that the receptor had a second function, possibly related to its ability to recycle from Golgi to ER. In this paper we investigate the requirements for Erd2p to support growth. We show that mutations that block its recycling also prevent growth. In addition, we show that all mutant receptors that can support growth have a residual ability to retrieve BiP, which is detectable when they are overexpressed. Mere recycling of an inactive form of the receptor, mediated by a cytoplasmic KKXX sequence, is not sufficient for growth. Furthermore, saturation of the receptor by expression of an HDEL-tagged version of pro-alpha factor inhibits growth, even of strains that do not show obvious BiP retention. We conclude that growth requires the HDEL-dependent retrieval of one or more proteins, and that these proteins can be recognized even under conditions where BiP is secreted. Genetic screens have failed to identify any one protein whose loss could account for the Erd2p requirement. Therefore, a growth may require the retention of multiple HDEL proteins in the ER, or alternatively the removal of such proteins from the Golgi apparatus.
Authors:
F M Townsley; G Frigerio; H R Pelham
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of cell biology     Volume:  127     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1994-10-31     Completed Date:  1994-10-31     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  21-8     Citation Subset:  IM    
Affiliation:
MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/X75780
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Fungal Proteins / metabolism*,  secretion
Genes, Fungal / genetics
Golgi Apparatus / metabolism
HSP70 Heat-Shock Proteins*
Heat-Shock Proteins / secretion
Membrane Proteins / genetics,  metabolism*
Models, Biological
Molecular Sequence Data
Mutation / physiology
Oligopeptides / genetics,  physiology*
Protein Sorting Signals / genetics
Receptors, Peptide / metabolism*,  physiology
Recombinant Fusion Proteins / metabolism
Saccharomyces cerevisiae / growth & development*,  metabolism
Saccharomyces cerevisiae Proteins*
Chemical
Reg. No./Substance:
0/ERD2 protein, S cerevisiae; 0/Fungal Proteins; 0/HDEL receptor; 0/HSP70 Heat-Shock Proteins; 0/Heat-Shock Proteins; 0/KAR2 protein, yeast; 0/Membrane Proteins; 0/Oligopeptides; 0/Protein Sorting Signals; 0/Receptors, Peptide; 0/Recombinant Fusion Proteins; 0/Saccharomyces cerevisiae Proteins; 129623-52-5/histidyl-aspartyl-glutamyl-leucine
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 10 Year: 1994
Volume: 127 Issue: 1
First Page: 21 Last Page: 28
ID: 2120172
Publisher Id: 95014711
PubMed Id: 7929564

Retrieval of HDEL proteins is required for growth of yeast cells


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