Document Detail

Retinol potentiates the inhibitory effect of ascorbic acid on uric acid assay.
MedLine Citation:
PMID:  20218904     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The interference of ascorbic acid in the determination of plasma uric acid concentrations, by decreasing the formation of chromophore in Trinder's reaction, is well known. By contrast, the effects of other antioxidant vitamins, such as retinol and alpha-tocopherol have not been investigated. Knowledge of the analytical interaction of these antioxidants in the uric acid assay would be useful. METHODS: The effect of a mix of vitamins (Cernevit) on uric acid analysis in a patient hospitalized in a neurological intensive care unit was observed. The effects of the different antioxidant components of the mixture (retinol, alpha-tocopherol and ascorbic acid) on the concentration of uric acid from a pool of plasma were also tested. RESULTS: Among the different combinations of vitamins tested, only retinol potentiated the antioxidant effect of ascorbic acid. The most marked effect was observed with 80 IU/mL of retinol and 5.7 mmol/L of ascorbic acid. CONCLUSIONS: We demonstrate for the first time the synergistic interference of ascorbic acid and retinol on the measurement of uric acid in plasma. We established that ascorbic acid interference is potentiated by regeneration of reduced ascorbic acid by retinol. Such interference must be kept in mind to avoid misinterpreting low plasma uric acid concentrations in patients in whom this analyte is critical.
Damien Bouvier; Vincent Sapin; Michèle Bonnard-Gougeon; Geoffroy Marceau
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical chemistry and laboratory medicine : CCLM / FESCC     Volume:  48     ISSN:  1434-6621     ISO Abbreviation:  Clin. Chem. Lab. Med.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-10     Completed Date:  2010-07-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9806306     Medline TA:  Clin Chem Lab Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  693-5     Citation Subset:  IM    
Department of Biochemistry, Clermont-Ferrand Teaching Hospital, Clermont-Ferrand, France.
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MeSH Terms
Antioxidants / pharmacology*
Ascorbic Acid / pharmacology*
Drug Synergism
Intensive Care Units
Uric Acid / blood*
Vitamin A / pharmacology*
Vitamins / pharmacology
alpha-Tocopherol / pharmacology
Reg. No./Substance:
0/Antioxidants; 0/Vitamins; 11103-57-4/Vitamin A; 50-81-7/Ascorbic Acid; 59-02-9/alpha-Tocopherol; 69-93-2/Uric Acid

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