| Retinol potentiates the inhibitory effect of ascorbic acid on uric acid assay. | |
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MedLine Citation:
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PMID: 20218904 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The interference of ascorbic acid in the determination of plasma uric acid concentrations, by decreasing the formation of chromophore in Trinder's reaction, is well known. By contrast, the effects of other antioxidant vitamins, such as retinol and alpha-tocopherol have not been investigated. Knowledge of the analytical interaction of these antioxidants in the uric acid assay would be useful. METHODS: The effect of a mix of vitamins (Cernevit) on uric acid analysis in a patient hospitalized in a neurological intensive care unit was observed. The effects of the different antioxidant components of the mixture (retinol, alpha-tocopherol and ascorbic acid) on the concentration of uric acid from a pool of plasma were also tested. RESULTS: Among the different combinations of vitamins tested, only retinol potentiated the antioxidant effect of ascorbic acid. The most marked effect was observed with 80 IU/mL of retinol and 5.7 mmol/L of ascorbic acid. CONCLUSIONS: We demonstrate for the first time the synergistic interference of ascorbic acid and retinol on the measurement of uric acid in plasma. We established that ascorbic acid interference is potentiated by regeneration of reduced ascorbic acid by retinol. Such interference must be kept in mind to avoid misinterpreting low plasma uric acid concentrations in patients in whom this analyte is critical. |
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Authors:
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Damien Bouvier; Vincent Sapin; Michèle Bonnard-Gougeon; Geoffroy Marceau |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinical chemistry and laboratory medicine : CCLM / FESCC Volume: 48 ISSN: 1434-6621 ISO Abbreviation: Clin. Chem. Lab. Med. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-10 Completed Date: 2010-07-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9806306 Medline TA: Clin Chem Lab Med Country: Germany |
Other Details:
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Languages: eng Pagination: 693-5 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Clermont-Ferrand Teaching Hospital, Clermont-Ferrand, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
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pharmacology* Ascorbic Acid / pharmacology* Drug Synergism Humans Intensive Care Units Uric Acid / blood* Vitamin A / pharmacology* Vitamins / pharmacology alpha-Tocopherol / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Vitamins; 11103-57-4/Vitamin A; 50-81-7/Ascorbic Acid; 59-02-9/alpha-Tocopherol; 69-93-2/Uric Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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