| Retinol palmitate counteracts oxidative injury during experimental septic shock. | |
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MedLine Citation:
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PMID: 11455740 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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INTRODUCTION: Retinols seem to be of clinical importance in ameliorating the clinical consequences of septic shock. These beneficial effects of retinols are suggested to be due to an antioxidant property. The present study was undertaken in order to confirm or rule out such an effect of retinol palmitate (RP) in experimental septic shock by measuring F2-isoprostanes and a major prostaglandin F2 alpha metabolite as indicators of oxidative injury and inflammatory response, respectively. MATERIALS AND METHODS: Fourteen anaesthetised pigs were randomly given an injection of RP (2.300 IU x kg-1) or the corresponding volume of vehicle. All pigs received a continuous infusion of E. coli endotoxin (10 micrograms x kg-1 x h-1). Blood samples were analysed for lipid peroxidation products (8-iso-PGF2 alpha), indicating free radical induced oxidative injury and 15-keto-dihydro-PGF2 alpha indicating cyclooxygenase-mediated inflammatory response). RESULTS: Significantly elevated levels of 8-iso-PGF2 alpha were seen at 3, 5 and 6 hours of endotoxaemia in the vehicle + endotoxin group as compared to RP + endotoxin group. Endotoxin induced cyclooxygenase-mediated inflammatory response was not affected by RP. CONCLUSIONS: This study is the first one to show that RP counteracts oxidative injury rather than inflammatory response in experimental septic shock. These results may be of importance for the understanding of some beneficial effects of RP during endotoxaemia (i.e. improved systemic haemodynamics and reduced serum levels of endotoxin). Our results may explain the therapeutic effects of nutrients rich in caroten/retinols used in some clinical studies. |
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Authors:
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S Basu; M Eriksson |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Annals of the Academy of Medicine, Singapore Volume: 30 ISSN: 0304-4602 ISO Abbreviation: Ann. Acad. Med. Singap. Publication Date: 2001 May |
Date Detail:
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Created Date: 2001-07-17 Completed Date: 2001-12-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7503289 Medline TA: Ann Acad Med Singapore Country: Singapore |
Other Details:
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Languages: eng Pagination: 265-9 Citation Subset: IM |
Affiliation:
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Section of Geriatrics, Faculty of Medicine, Uppsala University, Box 609, SE-751 25 Uppsala, Sweden. samar.basu@geriatrik.uu.se |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Antioxidants / therapeutic use* Dinoprost / analogs & derivatives*, blood Disease Models, Animal Endotoxins / adverse effects Escherichia coli Infections / chemically induced, drug therapy* F2-Isoprostanes / blood Female Inflammation / blood, drug therapy* Lipid Peroxidation / drug effects Male Oxidative Stress / drug effects* Radioimmunoassay Random Allocation Shock, Septic / blood, drug therapy* Swine* Vitamin A / analogs & derivatives*, therapeutic use* |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Endotoxins; 0/F2-Isoprostanes; 11103-57-4/Vitamin A; 27415-26-5/8-epi-prostaglandin F2alpha; 35850-13-6/15-ketoprostaglandin F2alpha; 551-11-1/Dinoprost; 67924-63-4/endotoxin, Escherichia coli; 79-81-2/retinol palmitate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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