| Retinol-binding protein 4 (RBP4) protein expression is increased in omental adipose tissue of severely obese patients. | |
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MedLine Citation:
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PMID: 19816414 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Visceral fat has been linked to insulin resistance and type 2 diabetes mellitus (T2DM); and emerging data links RBP4 gene expression in adipose tissue with insulin resistance. In this study, we examined RBP4 protein expression in omental adipose tissue obtained from 24 severely obese patients undergoing bariatric surgery, and 10 lean controls (4 males/6 females, BMI = 23.2 +/- 1.5 kg/m(2)) undergoing elective abdominal surgeries. Twelve of the obese patients had T2DM (2 males/10 females, BMI: 44.7 +/- 1.5 kg/m(2)) and 12 had normal glucose tolerance (NGT: 4 males/8 females, BMI: 47.6 +/- 1.9 kg/m(2)). Adipose RBP4, glucose transport protein-4 (GLUT4), and p85 protein expression were determined by western blot. Blood samples from the bariatric patients were analyzed for serum RBP4, total cholesterol, triglycerides, and glucose. Adipose RBP4 protein expression (NGT: 11.0 +/- 0.6; T2DM: 11.8 +/- 0.7; lean: 8.7 +/- 0.8 arbitrary units) was significantly increased in both NGT (P = 0.03) and T2DM (P = 0.005), compared to lean controls. GLUT4 protein was decreased in both NGT (P = 0.02) and T2DM (P = 0.03), and p85 expression was increased in T2DM subjects, compared to NGT (P = 0.03) and lean controls (P = 0.003). Regression analysis showed a strong correlation between adipose RBP4 protein and BMI for all subjects, as well as between adipose RBP4 and fasting glucose levels in T2DM subjects (r = 0.76, P = 0.004). Further, in T2DM, serum RBP4 was correlated with p85 expression (r = 0.68, P = 0.01), and adipose RBP4 protein trended toward an association with p85 protein (r = 0.55, P = 0.06). These data suggest that RBP4 may regulate adiposity, and p85 expression in obese-T2DM, thus providing a link to impaired insulin signaling and diabetes in severely obese patients. |
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Authors:
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Karen R Kelly; Sangeeta R Kashyap; Valerie B O'Leary; Jennifer Major; Philip R Schauer; John P Kirwan |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-10-08 |
Journal Detail:
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Title: Obesity (Silver Spring, Md.) Volume: 18 ISSN: 1930-7381 ISO Abbreviation: Obesity (Silver Spring) Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-29 Completed Date: 2010-09-02 Revised Date: 2011-04-28 |
Medline Journal Info:
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Nlm Unique ID: 101264860 Medline TA: Obesity (Silver Spring) Country: United States |
Other Details:
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Languages: eng Pagination: 663-6 Citation Subset: IM |
Affiliation:
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Department of Pathobiology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adiposity
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physiology* Blood Glucose / metabolism* Blood Proteins / metabolism Body Mass Index* Diabetes Mellitus, Type 2 / complications, metabolism* Female Gastric Bypass Glucose Transporter Type 4 / metabolism Humans Insulin Intra-Abdominal Fat / metabolism* Male Obesity / complications, metabolism*, surgery Omentum Retinol-Binding Proteins, Plasma / metabolism* Signal Transduction |
| Grant Support | |
ID/Acronym/Agency:
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AG12834/AG/NIA NIH HHS; K12RR023264/RR/NCRR NIH HHS; M01 RR018390-056219/RR/NCRR NIH HHS; RR018390/RR/NCRR NIH HHS; T32DK007319/DK/NIDDK NIH HHS; UL1RR024989/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/Blood Proteins; 0/Glucose Transporter Type 4; 0/RBP4 protein, human; 0/Retinol-Binding Proteins, Plasma; 11061-68-0/Insulin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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