Document Detail

Retinoids and cancer: antitumoral effects of ATRA, 9-cis RA and the new retinoid IIF on the HL-60 leukemic cell line.
MedLine Citation:
PMID:  12766334     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To compare the antitumoral effects of all-trans retinoic acid (ATRA) and 9-cis retinoic acid (9-cis RA) with those of 5-OH,11-O-hydrophenanthrene (IIF), a new derivative of retinoic acid. MATERIALS AND METHODS: The effect of retinoids was tested on cell line HL-60. Cell differentiation and apoptosis were evaluated by morphological and biochemical analysis as BCL-2 protein and by DNA fragmentation assay. The ability to activate retinoic acid receptors (RAR) and/or retinoid X receptors (RXR) and to modulate gene expression was determined by transactivation assay. RESULTS: With cell line HL-60, the antiproliferative effect of IIF was stronger than that of ATRA and 9-cis RA. Following retinoid treatment, cells appeared to differentiate and apoptotic cells were observed. The appearance of DNA laddering and a decrease in the amount of BCL-2 protein confirmed apoptosis. IIF transcriptionally activated RXR-gamma more than RAR-alpha. CONCLUSION: The findings indicate that IIF transcriptionally activates RXR-gamma preferentially, induces apoptosis and has a more antiproliferative activity than ATRA and 9-cis RA on cell line HL-60.
M Orlandi; B Mantovani; K Ammar; E Avitabile; P Dal Monte; G Bartolini
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medical principles and practice : international journal of the Kuwait University, Health Science Centre     Volume:  12     ISSN:  1011-7571     ISO Abbreviation:  Med Princ Pract     Publication Date:    2003 Jul-Sep
Date Detail:
Created Date:  2003-05-26     Completed Date:  2003-08-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8901334     Medline TA:  Med Princ Pract     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  164-9     Citation Subset:  IM    
Copyright Information:
Copyright 2003 S. Karger AG, Basel
Dipartimento di Biologia Evoluzionistica Sperimentale, Sezione di Microbiologia, Università di Bologna, Bologna, Italia.
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MeSH Terms
Antineoplastic Agents / pharmacology*
DNA Fragmentation
Dose-Response Relationship, Drug
HL-60 Cells / drug effects*
Phenanthrenes / pharmacology*
Proto-Oncogene Proteins c-bcl-2 / drug effects
Receptors, Retinoic Acid / drug effects,  genetics
Transcription, Genetic / drug effects
Tretinoin / analogs & derivatives*,  pharmacology*
Reg. No./Substance:
0/5-OH,11-O-hydrophenanthrene; 0/Antineoplastic Agents; 0/Phenanthrenes; 0/Proto-Oncogene Proteins c-bcl-2; 0/Receptors, Retinoic Acid; 302-79-4/Tretinoin; 5352-74-9/9,13-retinoic acid

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