| Retinoid regulation of cell differentiation in a series of human papillomavirus type 16-immortalized human cervical epithelial cell lines. | |
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MedLine Citation:
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PMID: 7532115 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Retinoids are important regulators of cervical epithelial cell differentiation and have been used in the treatment of cervical cancer. In the present study we evaluate the effects of retinoic acid on expression of biochemical markers of differentiation and expression of human papillomavirus reading frames encoding the early gene products E6 and E7 in normal and HPV16-immortalized cervical epithelial cell lines. Our results indicate that the differentiation markers cytokeratins K5 and K16 and transglutaminase type 1 are suppressed by all-trans-retinoic acid (RA). A marked concentration-dependent reduction in the level of of each mRNA is observed with maximal suppression at 1 microM. Each of the HPV16-immortalized cell lines (ECE16-1, ECE16-D1 and ECE16-D2) are more sensitive to the effects of RA than normal cells. The level of HPV16 transcript encoding E6/E7 is not significantly suppressed by 1 microM RA in ECE16-1 cells, but is suppressed in ECE16-D1 and ECE16-D2 cells. In addition, an increase in HPV transcripts encoding E6/E7 is observed at intermediate (10 and 100 nM) retinoic acid concentrations in ECE16-1 and ECE16-D2 cells, but not in ECE16-D1 cells. Our results show that retinoids regulate E6/E7 transcript levels in some cervical cell lines but not in others, suggesting that different cervical tumors may respond to retinoids via different mechanisms. |
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Authors:
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C K Choo; E A Rorke; R L Eckert |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Carcinogenesis Volume: 16 ISSN: 0143-3334 ISO Abbreviation: Carcinogenesis Publication Date: 1995 Feb |
Date Detail:
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Created Date: 1995-03-23 Completed Date: 1995-03-23 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8008055 Medline TA: Carcinogenesis Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 375-81 Citation Subset: IM |
Affiliation:
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Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, OH 44106-4970. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Animals Cell Differentiation / drug effects Cell Division / drug effects, physiology Cell Line Cervix Uteri / cytology*, drug effects*, virology DNA-Binding Proteins* Epithelial Cells Epithelium / drug effects, virology Female Genome, Viral Humans Keratins / metabolism Mice Oncogene Proteins, Viral / genetics Papillomaviridae / genetics* Reading Frames Repressor Proteins* Transcription, Genetic / drug effects Transfection Tretinoin / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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AR39750/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/E2 protein, Human papillomavirus type 16; 0/E6 protein, Human papillomavirus type 16; 0/Oncogene Proteins, Viral; 0/Repressor Proteins; 0/oncogene protein E7, Human papillomavirus type 16; 302-79-4/Tretinoin; 68238-35-7/Keratins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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