Document Detail


Retinoid regulation of cell differentiation in a series of human papillomavirus type 16-immortalized human cervical epithelial cell lines.
MedLine Citation:
PMID:  7532115     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Retinoids are important regulators of cervical epithelial cell differentiation and have been used in the treatment of cervical cancer. In the present study we evaluate the effects of retinoic acid on expression of biochemical markers of differentiation and expression of human papillomavirus reading frames encoding the early gene products E6 and E7 in normal and HPV16-immortalized cervical epithelial cell lines. Our results indicate that the differentiation markers cytokeratins K5 and K16 and transglutaminase type 1 are suppressed by all-trans-retinoic acid (RA). A marked concentration-dependent reduction in the level of of each mRNA is observed with maximal suppression at 1 microM. Each of the HPV16-immortalized cell lines (ECE16-1, ECE16-D1 and ECE16-D2) are more sensitive to the effects of RA than normal cells. The level of HPV16 transcript encoding E6/E7 is not significantly suppressed by 1 microM RA in ECE16-1 cells, but is suppressed in ECE16-D1 and ECE16-D2 cells. In addition, an increase in HPV transcripts encoding E6/E7 is observed at intermediate (10 and 100 nM) retinoic acid concentrations in ECE16-1 and ECE16-D2 cells, but not in ECE16-D1 cells. Our results show that retinoids regulate E6/E7 transcript levels in some cervical cell lines but not in others, suggesting that different cervical tumors may respond to retinoids via different mechanisms.
Authors:
C K Choo; E A Rorke; R L Eckert
Related Documents :
9052765 - Effects of all-trans-retinoic acid and 13-cis-retinoic acid on breast-cancer cell lines...
11342245 - Mutagenicity of bisphenol a and its suppression by interferon-alpha in human rsa cells.
2122465 - Differentiation of f9 embryonal carcinoma cells induced by the c-jun and activated c-ha...
21601355 - Comparative in vitro cytotoxicity study of carbon nanotubes and titania nanostructures ...
2534475 - Glycemia and immunohistochemical changes in the endocrine pancreas of the turtle chryse...
19638175 - Siaa and siad are essential for inducing autoaggregation as a specific response to dete...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Carcinogenesis     Volume:  16     ISSN:  0143-3334     ISO Abbreviation:  Carcinogenesis     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-03-23     Completed Date:  1995-03-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8008055     Medline TA:  Carcinogenesis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  375-81     Citation Subset:  IM    
Affiliation:
Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, OH 44106-4970.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Cell Differentiation / drug effects
Cell Division / drug effects,  physiology
Cell Line
Cervix Uteri / cytology*,  drug effects*,  virology
DNA-Binding Proteins*
Epithelial Cells
Epithelium / drug effects,  virology
Female
Genome, Viral
Humans
Keratins / metabolism
Mice
Oncogene Proteins, Viral / genetics
Papillomaviridae / genetics*
Reading Frames
Repressor Proteins*
Transcription, Genetic / drug effects
Transfection
Tretinoin / pharmacology*
Grant Support
ID/Acronym/Agency:
AR39750/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/E2 protein, Human papillomavirus type 16; 0/E6 protein, Human papillomavirus type 16; 0/Oncogene Proteins, Viral; 0/Repressor Proteins; 0/oncogene protein E7, Human papillomavirus type 16; 302-79-4/Tretinoin; 68238-35-7/Keratins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Development of bioactive functions in hydrangeae dulcis folium. III. On the antiallergic and antimic...
Next Document:  A comparative study of the biochemical properties of human and mouse recombinant O6-methylguanine-DN...