Document Detail

Retinoid regulation of cell differentiation in a series of human papillomavirus type 16-immortalized human cervical epithelial cell lines.
MedLine Citation:
PMID:  7532115     Owner:  NLM     Status:  MEDLINE    
Retinoids are important regulators of cervical epithelial cell differentiation and have been used in the treatment of cervical cancer. In the present study we evaluate the effects of retinoic acid on expression of biochemical markers of differentiation and expression of human papillomavirus reading frames encoding the early gene products E6 and E7 in normal and HPV16-immortalized cervical epithelial cell lines. Our results indicate that the differentiation markers cytokeratins K5 and K16 and transglutaminase type 1 are suppressed by all-trans-retinoic acid (RA). A marked concentration-dependent reduction in the level of of each mRNA is observed with maximal suppression at 1 microM. Each of the HPV16-immortalized cell lines (ECE16-1, ECE16-D1 and ECE16-D2) are more sensitive to the effects of RA than normal cells. The level of HPV16 transcript encoding E6/E7 is not significantly suppressed by 1 microM RA in ECE16-1 cells, but is suppressed in ECE16-D1 and ECE16-D2 cells. In addition, an increase in HPV transcripts encoding E6/E7 is observed at intermediate (10 and 100 nM) retinoic acid concentrations in ECE16-1 and ECE16-D2 cells, but not in ECE16-D1 cells. Our results show that retinoids regulate E6/E7 transcript levels in some cervical cell lines but not in others, suggesting that different cervical tumors may respond to retinoids via different mechanisms.
C K Choo; E A Rorke; R L Eckert
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Carcinogenesis     Volume:  16     ISSN:  0143-3334     ISO Abbreviation:  Carcinogenesis     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-03-23     Completed Date:  1995-03-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8008055     Medline TA:  Carcinogenesis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  375-81     Citation Subset:  IM    
Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, OH 44106-4970.
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MeSH Terms
3T3 Cells
Cell Differentiation / drug effects
Cell Division / drug effects,  physiology
Cell Line
Cervix Uteri / cytology*,  drug effects*,  virology
DNA-Binding Proteins*
Epithelial Cells
Epithelium / drug effects,  virology
Genome, Viral
Keratins / metabolism
Oncogene Proteins, Viral / genetics
Papillomaviridae / genetics*
Reading Frames
Repressor Proteins*
Transcription, Genetic / drug effects
Tretinoin / pharmacology*
Grant Support
Reg. No./Substance:
0/DNA-Binding Proteins; 0/E2 protein, Human papillomavirus type 16; 0/E6 protein, Human papillomavirus type 16; 0/Oncogene Proteins, Viral; 0/Repressor Proteins; 0/oncogene protein E7, Human papillomavirus type 16; 302-79-4/Tretinoin; 68238-35-7/Keratins

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