| Retinoid processing in cone and Müller cell lines. | |
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MedLine Citation:
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PMID: 18163989 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To determine whether cones and Müller cells in the rod dominated retina cooperate to regenerate the 11-cis retinal chromophore via the retinoid cycle, two cell lines from the rod dominated retinas of Murine were used for this study: 661W, a mouse cell line derived from cones, and rMC-1, a rat Müller cell line. Retinoid cycle enzymes were analyzed by RT-PCR, and their catalytic activity was detected by incubation with retinoids and analyzed by HPLC. We found that 661W cells are capable of reducing all-trans retinal to all-trans retinol due to the presence of multiple dehydrogenases and to generate minor amounts of retinyl-ester. The rMC-1 cells take up all-trans retinol and oxidize it to all-trans retinal or esterify it to retinyl-ester, but are incapable of isomerizing all-trans retinoids to 11-cis retinoids. This could be a reflection of lack of necessary activities in Müller cells in vivo, which suggests that Müller cells do not contribute to retinoid cycling by regenerating 11-cis retinoids. Alternatively, this could be due to the potential that rMC-1, as a transformed cell line, has stopped expressing the proteins needed for the regeneration of 11-cis retinoids. |
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Authors:
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Yogita Kanan; Anne Kasus-Jacobi; Gennadiy Moiseyev; Kjell Sawyer; Jian-Xing Ma; Muayyad R Al-Ubaidi |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-11-17 |
Journal Detail:
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Title: Experimental eye research Volume: 86 ISSN: 0014-4835 ISO Abbreviation: Exp. Eye Res. Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-02-18 Completed Date: 2008-04-16 Revised Date: 2010-12-03 |
Medline Journal Info:
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Nlm Unique ID: 0370707 Medline TA: Exp Eye Res Country: England |
Other Details:
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Languages: eng Pagination: 344-54 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Chromatography, High Pressure Liquid / methods Esters / metabolism Mice Mice, Inbred C57BL Oxidation-Reduction Oxidoreductases / physiology Phosphatidylcholine-Sterol O-Acyltransferase / metabolism Photic Stimulation Retina / metabolism* Retinal Cone Photoreceptor Cells / metabolism Retinaldehyde / metabolism Retinoids / metabolism* Retinol O-Fatty-Acyltransferase / metabolism Reverse Transcriptase Polymerase Chain Reaction / methods Vitamin A / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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EY 015650/EY/NEI NIH HHS; P20 RR 017703/RR/NCRR NIH HHS; P30 EY 12190/EY/NEI NIH HHS; R01 EY 012231/EY/NEI NIH HHS; R01 EY 14052/EY/NEI NIH HHS; R01 EY014052-04/EY/NEI NIH HHS; R01 EY018137-01A2/EY/NEI NIH HHS; R01 EY018137-02/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Esters; 0/Retinoids; 11103-57-4/Vitamin A; 116-31-4/Retinaldehyde; EC 1.-/Oxidoreductases; EC 2.3.1.43/Phosphatidylcholine-Sterol O-Acyltransferase; EC 2.3.1.76/Retinol O-Fatty-Acyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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