Document Detail


Retinoid agonist activities of synthetic geranyl geranoic acid derivatives.
MedLine Citation:
PMID:  7726866     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Micromolar concentrations of 4,5- didehydro geranyl geranoic acid (GGA) were able to induce up-regulation of retinoic acid receptor-beta gene expression in human hepatoma-derived cell line, HuH-7, to the same extent as all-trans RA. In chloramphenicol acetyl transferase (CAT) assay with retinoic acid response element-beta, GGA and 4,5-didehydro GGA were both positive, but 2,3-dihydro GGA was negative, even though these GGA derivatives have been reported to be all potent ligands for cellular retinoic-acid-binding protein(CRABP). However, 10,11,14,15- tetrahydro- 4,5- didehydro GGA, a compound without any affinity for CRABP, transactivated CAT gene expression. On the other hand, only GGA and 4,5-didehydro GGA were active to induce CAT gene expression through retinoid X response element of cellular retinol binding protein, type II gene. We show for the first time that chemically synthesized acyclic organic acids are potential agonists of natural retinoids.
Authors:
H Araki; Y Shidoji; Y Yamada; H Moriwaki; Y Muto
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  209     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-05-25     Completed Date:  1995-05-25     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  66-72     Citation Subset:  IM    
Affiliation:
First Department of Internal Medicine, Gifu University School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Chloramphenicol O-Acetyltransferase / genetics
Diterpenes / chemistry,  pharmacology*
Gene Expression Regulation / drug effects
Humans
Receptors, Retinoic Acid / genetics
Retinoids / agonists*,  pharmacology
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Diterpenes; 0/Receptors, Retinoic Acid; 0/Retinoids; 0/retinoic acid binding protein II, cellular; 0/retinoic acid receptor beta; 83807-40-3/geranylgeranic acid; EC 2.3.1.28/Chloramphenicol O-Acetyltransferase

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