Document Detail


Retinoic acid suppresses parathyroid hormone (PTH) secretion and PreproPTH mRNA levels in bovine parathyroid cell culture.
MedLine Citation:
PMID:  8113407     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1,25-dihydroxyvitamin D3[1,25(OH)2D3] suppresses parathyroid hormone (PTH) gene transcription. Recent evidence suggests that retinoid X receptors are involved in 1,25(OH)2D3-mediated transcriptional events. However, little data exists for a role of retinoids in parathyroid function or in PTH expression. In the present study, we observed that all-trans- or 9-cis retinoic acid suppressed the release of PTH from bovine parathyroid cell cultures. Both retinoids were remarkably potent with significant decreases evident at 10(-10) M and a maximally suppressive effect (approximately 65%) at 10(-7) M. All-trans-retinol was considerably less potent in this system. The effect was not evident until 12 h, suggesting that retinoids did not affect the rapid secretion of preexisting PTH stores. PreproPTH mRNA levels were also suppressed by retinoic acid and the retinoid potencies were similar to those observed in the secretion studies. Combined treatment with 10(-6) M retinoic acid and 10(-8) M 1,25(OH)2D3 more effectively decreased PTH secretion and preproPTH mRNA than did either compound alone. These data indicate that retinoic acid: (a) elicits a bioresponse in bovine parathyroid cells; (b) attenuates PTH expression at the protein and mRNA levels, and (c) acts independently of 1,25(OH)2D3 in the control of PTH expression.
Authors:
P N MacDonald; C Ritter; A J Brown; E Slatopolsky
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  93     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-03-30     Completed Date:  1994-03-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  725-30     Citation Subset:  AIM; IM    
Affiliation:
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Northern
Calcitriol / pharmacology
Cattle
Cells, Cultured
Isomerism
Kinetics
Parathyroid Glands / drug effects*,  metabolism,  secretion
Parathyroid Hormone / biosynthesis*,  secretion*
Protein Precursors / biosynthesis*
RNA, Messenger / metabolism*
Retinoids / pharmacology
Tretinoin / pharmacology*
Grant Support
ID/Acronym/Agency:
DK-07126/DK/NIDDK NIH HHS; DK-09976/DK/NIDDK NIH HHS; DK-30178/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Parathyroid Hormone; 0/Protein Precursors; 0/RNA, Messenger; 0/Retinoids; 302-79-4/Tretinoin; 32222-06-3/Calcitriol; 67418-29-5/preproparathormone
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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