| Retinoic acid stimulates essential fatty acid-supplemented human keratinocytes in culture. | |
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MedLine Citation:
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PMID: 9129229 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of all-trans retinoic acid on the proliferation of essential fatty acid (EFA)-deficient and of EFA-supplemented adult human keratinocytes was investigated. EFA-deficient cell strains were supplied with one of four different fatty acid-supplemented media at the P0 to P1 passage. All-trans retinoic acid at 0.5 or 1.0 microM was added to the cultures at the P1 to P2 passage. At passage P3, and 3 and 7 d thereafter, the cell growth rate was determined. The fatty acid content of cultures grown in each medium was measured using gas chromatography. All the EFA media "normalized" the cellular fatty acid composition and drastically decreased the cell number and total DNA and protein of the cultures. All-trans retinoic acid at 1 microM prevented the loss of cell viability and growth usually associated with EFA supplementation but did not affect the control (EFA deficient) or 18:1 fatty acid-supplemented cultures. All-trans retinoic acid at 1 microM altered the fatty acid content of the EFA-supplemented cultures. A statistically significant increase in 14:0, 14:1, 16:1, 18:1, and 20:4 fatty acids occurred, whereas the amounts of 18:0 and 18:2 fatty acids decreased. The largest changes were in 16:1 fatty acid (8-14%) and 18:2 fatty acid (12-5%). All-trans retinoic acid at 0.5 microM also affected both cell growth and fatty acid composition without induction of the CRABP II message. These studies demonstrate that all-trans retinoic acid stimulates the growth of EFA-supplemented keratinocyte cultures while also altering the fatty acid composition of the cells. |
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Authors:
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C L Marcelo; W R Dunham |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of investigative dermatology Volume: 108 ISSN: 0022-202X ISO Abbreviation: J. Invest. Dermatol. Publication Date: 1997 May |
Date Detail:
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Created Date: 1997-05-13 Completed Date: 1997-05-13 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0426720 Medline TA: J Invest Dermatol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 758-62 Citation Subset: IM |
Affiliation:
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Department of Surgery, University of Michigan Medical School, Ann Arbor 48109-0592, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Cell Count / drug effects Cell Division / genetics Cells, Cultured DNA / analysis Dose-Response Relationship, Drug Fatty Acids, Essential / pharmacology* Humans Keratinocytes / chemistry, cytology*, drug effects Receptors, Retinoic Acid / physiology Time Factors Tretinoin / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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AR 26009/AR/NIAMS NIH HHS; AR 42223/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Essential; 0/Receptors, Retinoic Acid; 0/retinoic acid binding protein II, cellular; 302-79-4/Tretinoin; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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