Document Detail

Retinoic acid receptor beta is required for anti-activator protein-1 activity by retinoic acid in gastric cancer cells.
MedLine Citation:
PMID:  12123542     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To investigate the role of retinoic acid receptor beta (RARbeta) in mediating inhibitory effect of all-trans retinoic acid (ATRA) on activator protein-1 (AP-1) activity in gastric cancer cells. METHODS: Transient transfection and chloramphenicol acetyltransferase (CAT) assay, Nort hern blot, gene transfection, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, and anchorage independent growth assay were used. RESULTS: Transient transfection of RARbeta expression vector into MKN-45 cells resulted in the RARbeta concentration dependent repression of AP-1 activity induced by 12-o-tetradecanoylphorbol-13-acetate (TPA), regardless of the presence of ATRA. When the c-jun and c-fos expression vectors were cotransfected with the RARbeta expression vector into MKN-45 cells, AP-1 activity was also obviously repressed. The inhibitory effect, again, was RARbeta-concentration-dependent. The stable transfection of the RARbeta gene into MKN-45 cells led to cell growth inhibition and colony formation inhibition by ATRA. Furthermore, Cotransfection of both RARbeta/DNA binding domain (DBD) and reporter gene could not alter AP-1 activity, even in the presence of ATRA.However, when the cotransfection was substituted with the RARbeta/ligand binding domain (LBD), the inhibition was significantly enhanced by ATRA. CONCLUSION: RARbeta might be required for anti-AP-1 activity, and contribute to growth inhibition of gastric cancer cells by ATRA.
Qiao Wu; Mingqing Zhang; Su Liu; Yuqiang Chen; Wenjin Su
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chinese medical journal     Volume:  115     ISSN:  0366-6999     ISO Abbreviation:  Chin. Med. J.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-07-18     Completed Date:  2002-08-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7513795     Medline TA:  Chin Med J (Engl)     Country:  China    
Other Details:
Languages:  eng     Pagination:  810-4     Citation Subset:  IM    
Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, the School of Life Sciences, Xiamen University, China.
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MeSH Terms
Antineoplastic Agents / pharmacology*
Binding Sites
Cell Division / drug effects
DNA / metabolism
Receptors, Retinoic Acid / chemistry,  physiology*
Stomach Neoplasms / drug therapy*,  pathology
Transcription Factor AP-1 / antagonists & inhibitors*
Tretinoin / pharmacology*
Tumor Cells, Cultured
Reg. No./Substance:
0/Antineoplastic Agents; 0/Receptors, Retinoic Acid; 0/Transcription Factor AP-1; 0/retinoic acid receptor beta; 302-79-4/Tretinoin; 9007-49-2/DNA

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