| Retinoic acid receptor (RAR)-alpha is not critically required for mediating retinoic acid effects in the developing mouse retina. | |
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MedLine Citation:
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PMID: 20107170 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To determine the functional contribution of retinoic acid receptor (RAR)-alpha in the developing murine neural retina, through a phenotypic analysis of the corresponding null mutants. METHODS: RARalpha mutant (Rara(-/-)) mice were compared with wild-type littermates at several stages of pre- and postnatal development. An RA-response element (RARE)-containing reporter transgene was used to assess the contribution of RARalpha to retinoid signaling in the retina. In situ hybridization was performed on serial eye sections to investigate the expression of main developmental regulators. Immunofluorescence was used to detect differentiated cell types in the adult retina. Mutants were also subjected to clinical observation and visual function evaluation with the optomotor test and electroretinography. RESULTS: Both isoform transcripts of RARalpha were expressed throughout the neural retina at various stages of pre- and postnatal development. In the Rara(-/-) mice the RARE-reporter transgene consistently failed to activate in the developing neural retina. However, they did not exhibit any alteration of the expression patterns of molecular determinants and had a normal organization of retinal cell types at postnatal stages. Their performance in visual tests was indistinguishable from that of control littermates. CONCLUSIONS: Although RARalpha mediates RARE reporter transgene activity in the neural retina, its function is not necessary for the retina to develop and function normally. These data suggest that retinoic acid regulates neural retinal development through other, possibly RAR-independent, pathways. |
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Authors:
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Laura Cammas; Fr?d?ric Trensz; Abdeljalil Jellali; Norbert B Ghyselinck; Michel J Roux; Pascal Doll? |
Publication Detail:
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Type: Journal Article Date: 2010-01-27 |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 51 ISSN: 1552-5783 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-20 Completed Date: 2010-06-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: United States |
Other Details:
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Languages: eng Pagination: 3281-90 Citation Subset: IM |
Affiliation:
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Institut de G?n?tique et de Biologie Mol?culaire et Cellulaire, BP 10142 Illkirch, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation Electroretinography Embryonic Development / physiology Fluorescein Angiography Fluorescent Antibody Technique, Indirect Gene Expression Regulation / physiology Genes, Reporter In Situ Hybridization Mice Mice, Knockout Mice, Transgenic Motor Activity Protein Isoforms Receptors, Retinoic Acid / physiology* Response Elements Retina / embryology*, metabolism Tretinoin / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Protein Isoforms; 0/Receptors, Retinoic Acid; 0/retinoic acid receptor alpha; 302-79-4/Tretinoin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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