Document Detail

Retinoic acid perturbs the expression of Xhox.lab genes and alters mesodermal determination in Xenopus laevis.
MedLine Citation:
PMID:  1678362     Owner:  NLM     Status:  MEDLINE    
Retinoic acid (RA) treatment of Xenopus laevis embryos leads to anterior truncation of the body axis (Durston et al. 1989; Sive et al. 1990). These initial studies suggested that RA may play a role in the patterning of the primary body axis. At least one target of RA was shown previously to be dorsal ectoderm. In this report we extend this observation and also ask whether RA alters the determination and inducing capacity of mesodermal tissue. To facilitate this analysis we isolated the homeo-domain-containing genes Xhox.lab1 and Xhox.lab2. These genes were expressed in both ectoderm and mesoderm during the RA-sensitive period and were strongly induced by RA in both germ layers. In particular, anterior regions expressed low levels of Xhox.lab RNAs in untreated embryos but showed increased expression after RA application. We show further that although RA-treated embryos contained anterior-inducing mesoderm, the amount of this activity appeared to be lower than that of controls. Additionally, we document that RA suppressed lateral (muscle) and ventral (blood) mesoderm differentiation. The data indicate that RA alters mesodermal determination and causes axial perturbation both by depressing the ability of dorsal mesoderm to induce anterior structures and by altering the response of dorsal ectoderm to induction. These analyses suggest that Xhox.lab genes may be responsible, in part, for mediating the RA effect.
H L Sive; P F Cheng
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genes & development     Volume:  5     ISSN:  0890-9369     ISO Abbreviation:  Genes Dev.     Publication Date:  1991 Aug 
Date Detail:
Created Date:  1991-09-13     Completed Date:  1991-09-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8711660     Medline TA:  Genes Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1321-32     Citation Subset:  IM    
Department of Genetics, Fred Hutchinson Cancer Center, Seattle, Washington 98104.
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MeSH Terms
Amino Acid Sequence
Blotting, Northern
Ectoderm / drug effects,  physiology
Embryo, Nonmammalian / drug effects,  physiology*,  radiation effects
Gene Expression / drug effects,  radiation effects
Genes, Homeobox / drug effects*
Mesoderm / drug effects,  physiology*,  radiation effects
Molecular Sequence Data
Multigene Family*
Sequence Homology, Nucleic Acid
Tretinoin / pharmacology*
Ultraviolet Rays
Xenopus laevis
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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