Document Detail


Retinoic acid and nitric oxide promote cell proliferation and differentially induce neuronal differentiation in vitro in the cnidarian Renilla koellikeri.
MedLine Citation:
PMID:  20635350     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Retinoic acid (RA) and nitric oxide (NO) are known to promote neuronal development in both vertebrates and invertebrates. Retinoic acid receptors appear to be present in cnidarians and NO plays various physiological roles in several cnidarians, but there is as yet no evidence that these agents have a role in neural development in this basal metazoan phylum. We used primary cultures of cells from the sea pansy Renilla koellikeri to investigate the involvement of these signaling molecules in cnidarian cell differentiation. We found that 9-cis RA induce cell proliferation in dose- and time-dependent manners in dishes coated with polylysine from the onset of culture. Cells in cultures exposed to RA in dishes devoid of polylysine were observed to differentiate into epithelium-associated cells, including sensory cells, without net gain in cell density. NO donors also induce cell proliferation in polylysine-coated dishes, but induce neuronal differentiation and neurite outgrowth in uncoated dishes. No other cell type undergoes differentiation in the presence of NO. These observations suggest that in the sea pansy (1) cell adhesion promotes proliferation without morphogenesis and this proliferation is modulated positively by 9-cis RA and NO, (2) 9-cis RA and NO differentially induce neuronal differentiation in nonadherent cells while repressing proliferation, and (3) the involvement of RA and NO in neuronal differentiation appeared early during the evolutionary emergence of nervous systems.
Authors:
Djoyce Estephane; Michel Anctil
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental neurobiology     Volume:  70     ISSN:  1932-846X     ISO Abbreviation:  Dev Neurobiol     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-16     Completed Date:  2011-01-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101300215     Medline TA:  Dev Neurobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  842-52     Citation Subset:  IM    
Copyright Information:
2010 Wiley Periodicals, Inc.
Affiliation:
Département de sciences biologiques and Centre de recherche en sciences neurologiques, Université de Montréal, Case postale 6128, Succursale Centre-ville, Montréal, Québec, Canada, H3C 3J7.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion / drug effects
Cell Differentiation / drug effects*
Cell Proliferation / drug effects*
Cells, Cultured
Epithelial Cells / cytology,  drug effects
Neurites / drug effects
Neurons / drug effects*
Nitric Oxide / pharmacology*
Renilla / cytology*
Tretinoin / pharmacology*
Chemical
Reg. No./Substance:
10102-43-9/Nitric Oxide; 302-79-4/Tretinoin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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