Document Detail


Retinoic acid involvement in the reciprocal neurotrophic interactions between newt spinal cord and limb blastemas in vitro.
MedLine Citation:
PMID:  12524177     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to investigate the reciprocal neurotrophic interaction between regenerating limb blastemas and spinal cord explants from the newt Notophthalmus viridescens. Axon outgrowth was measured from spinal cord explants in vitro to assess the neurotrophic activity of early to mid-bud stage blastemas after various treatments. When retinoic acid, a vitamin A metabolite, was added to the medium, it increased both the number and length of axons extending from spinal cord explants. Spinal cord explants co-cultured with blastemas that were previously treated with citral, an inhibitor of retinoic acid synthesis, extended significantly fewer axons than control co-cultures. Blastemas, which were denervated by surgical resection of the brachial plexus 48 h before co-culture, also exhibited a significantly weaker neurotrophic activity than did control innervated blastemas. These results are consistent with a reciprocal interaction between blastema mesenchyme and nerves and suggest either a stimulatory or synergistic role for endogenous retinoic acid in the blastema-derived trophic activity.
Authors:
David J Prince; Robert L Carlone
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research. Developmental brain research     Volume:  140     ISSN:  0165-3806     ISO Abbreviation:  Brain Res. Dev. Brain Res.     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2003-01-13     Completed Date:  2003-03-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8908639     Medline TA:  Brain Res Dev Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  67-73     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Brock University, 500 Glenridge Avenue, St. Catharines, Ontario L2S 3A1, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Denervation
Ethanol / pharmacology
Forelimb / drug effects,  innervation*
Mitosis
Monoterpenes / pharmacology
Nerve Regeneration / drug effects,  physiology*
Neurons / cytology,  drug effects,  physiology*
Organ Culture Techniques
Salamandridae
Spinal Cord / cytology,  drug effects,  physiology*
Tretinoin / pharmacology*
Chemical
Reg. No./Substance:
0/Monoterpenes; 302-79-4/Tretinoin; 5392-40-5/citral; 64-17-5/Ethanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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