Document Detail

Retinoic acid induces myoblasts transdifferentiation into premeiotic Stra8-positive cells.
MedLine Citation:
PMID:  21091440     Owner:  NLM     Status:  MEDLINE    
Spermatogonia and sperm-like cells can be derived in vitro via the addition of RA (retinoic acid) to pluripotent ES and EG cells. At present, however, these cells have not been derived from unipotent cells. Here, we have generated premeiotic Stra8-positive cells from C2C12 myoblasts following treatment with 10 μM all-trans-RA for 8 days. The differentiated C2C12 cells exhibited spherical morphology similar to spermatogonia, and they expressed gene markers of premeiosis, meiosis and postmeiosis. In addition, some of the transdifferentiated Stra8-positive cells had a tail-like phenotype. Flow cytometry results indicated that up to 20% of RA-induced C2C12 cells were Stra8-positive. Mvh (mouse vasa homologue) protein, a germ cell-specific ATP-dependent RNA helicase and Prm1 (protamine 1) were detected in transdifferentiated cells. The DNA content in induced C2C12 cells showed that Stra8-positive cells were diploid, suggesting that the myoblast transdifferentiation was in the premeiotic stage of spermatogenesis. The derivation of Stra8-positive cells from C2C12 myoblasts has important implications for studying unipotent cell differentiation. Furthermore, C2C12 myoblasts may provide a useful in vitro cell model to study signal transduction and transdifferentiation during RA treatments.
Wenwen Jia; De Cheng; Shuai Chen; Lei Lei; Huayan Wang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  35     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-18     Completed Date:  2011-07-14     Revised Date:  2012-08-02    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  365-72     Citation Subset:  IM    
College of Veterinary Medicine, Shaanxi Center for Stem Cell Engineering and Technology, Yangling, Shaanxi 712100, Peoples Republic of China.
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MeSH Terms
Cell Line
Cell Line, Tumor
Cell Transdifferentiation*
Gene Expression
Myoblasts / cytology,  metabolism*
NIH 3T3 Cells
Proteins / genetics,  metabolism*
Spermatogonia / cytology,  metabolism*
Tretinoin / metabolism*
Reg. No./Substance:
0/Proteins; 0/Stra8 protein, mouse; 302-79-4/Tretinoin

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