Document Detail

Retinoic acid enhances cell responses to epidermal growth factor in mouse mammary gland in culture.
MedLine Citation:
PMID:  3512256     Owner:  NLM     Status:  MEDLINE    
Addition of epidermal growth factor (EGF) at up to 100 ng/ml to the medium of cultured explants of mouse mammary gland increased thymidine incorporation into DNA dose dependently. Addition of retinoic acid alone at 10 micrograms/ml to the medium had no significant effect on DNA synthesis, but addition of EGF with retinoic acid enhanced the EGF-stimulated DNA synthesis. Furthermore, pretreatment of mammary explants with retinoic acid enhanced the effect of EGF plus retinoic acid on cell growth. EGF inhibited the synthesis of casein and decreased alpha-lactalbumin activity of mammary explants in culture in the presence of insulin, cortisol, and PRL. Retinoic acid alone had no significant effect on the synthesis of casein, but suppressed alpha-lactalbumin activity dose dependently. Concomitant addition of retinoic acid with EGF had no significant effect on EGF-induced inhibition of casein synthesis, but enhanced EGF-induced inhibition of alpha-lactalbumin activity dose dependently. Measurement of specific binding of [125I]EGF to mouse mammary glands in culture demonstrated that pretreatment of the explants with retinoic acid slightly, but significantly, enhanced the specific binding of EGF to its cellular receptors.
H Komura; H Wakimoto; C F Chen; N Terakawa; T Aono; O Tanizawa; K Matsumoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrinology     Volume:  118     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1986 Apr 
Date Detail:
Created Date:  1986-04-24     Completed Date:  1986-04-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1530-6     Citation Subset:  AIM; IM    
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MeSH Terms
Caseins / biosynthesis
Cell Survival
Cells, Cultured
DNA Replication / drug effects
Dose-Response Relationship, Drug
Drug Synergism
Epidermal Growth Factor / pharmacology*
Hydrocortisone / pharmacology
Insulin / pharmacology
Lactalbumin / metabolism
Mammary Glands, Animal / cytology,  drug effects*
Mice, Inbred C3H
Prolactin / pharmacology
Thymidine / metabolism
Tretinoin / pharmacology*
Reg. No./Substance:
0/Caseins; 11061-68-0/Insulin; 302-79-4/Tretinoin; 50-23-7/Hydrocortisone; 50-89-5/Thymidine; 62229-50-9/Epidermal Growth Factor; 9002-62-4/Prolactin; 9013-90-5/Lactalbumin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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