Document Detail

Retinoic acid decreases the viability of mouse blastocysts in vitro.
MedLine Citation:
PMID:  12525453     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: This study was designed to examine the cytotoxic effect of retinoic acid on the blastocyst stage of mouse embryos and on subsequent early postimplantation embryo development in vitro. METHODS AND RESULTS: Mouse blastocysts were exposed for 24 h to doses of 0, 0.1 micromol/l and 10 micromol/l all-trans retinoic acid and observed for their capacity to implant and develop during the early postimplantation period in vitro. When retinoic acid-pretreated blastocysts were allowed to implant in vitro, significantly fewer embryos were able to reach a later stage of embryo development. Compared with the findings for the control blastocysts, exposure to retinoic acid resulted in a significant reduction in the average number of total cells in blastocysts and the trophectoderm/inner cell mass lineage. The effect was associated with a significant increase in the frequency of cells identified as being engaged in apoptosis by means of the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling and Annexin V techniques. CONCLUSIONS: This is the first evidence that retinoic acid induces cell death (apoptosis) and inhibits cell proliferation in mouse blastocysts. This results in the retardation of early postimplantation blastocyst development and subsequent blastocyst death.
Fu-Jen Huang; Chung-Chang Shen; Shiuh-Young Chang; Tsung-Chieh J Wu; Yan-Der Hsuuw
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  18     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2003-01-14     Completed Date:  2003-07-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  130-6     Citation Subset:  IM    
Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Kaohsiung, Taiwan.
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MeSH Terms
Blastocyst / cytology,  drug effects*,  physiology*
Cell Division / drug effects
Cell Survival / drug effects
Culture Techniques
Embryo Implantation
Embryonic and Fetal Development / drug effects
Mice, Inbred ICR
Tretinoin / pharmacology*
Reg. No./Substance:

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