Document Detail

Retinoic acid and 12-O-tetradecanoylphorbol-13-acetate alter release of glycoproteins from mouse fibroblast BALB/C 3T6 cells.
MedLine Citation:
PMID:  3919954     Owner:  NLM     Status:  MEDLINE    
Treatment of mouse fibroblast BALB/c 3T6 cells with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate or the antipromoter retinoic acid affects the release of several glycoproteins into the medium. The phorbol ester decreases the secretion of a 180-kd and 160-kd glycoprotein and increases the release of a 38-kd glycoprotein. In contrast, retinoic acid affects these glycoproteins in the opposite way. Moreover, retinoic acid enhances the level of a 55-kd and 60-kd glycoprotein. The 180-kd and 160-kd glycoproteins appear sensitive to collagenase and after pepsin treatment are converted to bands which comigrate with collagen alpha 1 (I) and alpha 2 (I). These glycoproteins are tentatively identified as being pro alpha 1 (I) and pro alpha 2 (I). The 38-kd glycoprotein appears to comigrate with the major excreted protein. Retinoic acid appears to reduce significantly the incorporation of mannose into secreted glycoproteins whereas treatment with the phorbol ester induces an enhancement in mannose incorporation. Our results indicate that both phorbol esters and retinoids alter the release of several glycoproteins from 3T6 mouse fibroblasts. These changes appear to relate to the influence of these compounds on the expression of the transformed phenotype of these cells.
A M Jetten; L M De Luca
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Carcinogenesis     Volume:  6     ISSN:  0143-3334     ISO Abbreviation:  Carcinogenesis     Publication Date:  1985 Mar 
Date Detail:
Created Date:  1985-04-30     Completed Date:  1985-04-30     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8008055     Medline TA:  Carcinogenesis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  337-42     Citation Subset:  IM    
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MeSH Terms
Amino Acids / metabolism
Cells, Cultured
Collagen / biosynthesis
Fibroblasts / drug effects,  metabolism
Glycoproteins / secretion*
Mice, Inbred BALB C
Molecular Weight
Pepsin A / pharmacology
Phorbols / toxicity*
Tetradecanoylphorbol Acetate / toxicity*
Tretinoin / pharmacology*
Reg. No./Substance:
0/Amino Acids; 0/Glycoproteins; 0/Phorbols; 16561-29-8/Tetradecanoylphorbol Acetate; 302-79-4/Tretinoin; 9007-34-5/Collagen; EC A

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