Document Detail

Retinoblastoma-related protein pRb2/p130 and its binding to the B-myb promoter increase during human neuroblastoma differentiation.
MedLine Citation:
PMID:  9361185     Owner:  NLM     Status:  MEDLINE    
Neuroblastoma cells can undergo neural differentiation upon treatment with a variety of chemical inducers and growth factors. During this process, many cell cycle-related genes are downregulated while differentiation-specific genes are triggered. The retinoblastoma family proteins, pRb, p107, and pRb2/p130, are involved in transcriptional repression of proliferation genes, mainly through their interaction with the E2F transcription factors. We report that pRb2/p130 expression levels increased during differentiation of neuroblastoma cell line LAN-5. On the other hand, both pRb and p107 decreased and underwent progressive dephosphorylation at late differentiation times. The expression of B-myb and c-myb, two targets of the retinoblastoma family proteins, were downregulated in association with the increase of pRb2/p130, which was detected as the major component of the complex with E2F on the E2F site of the B-myb promoter in differentiated cells. Interestingly, E2F4, a preferential partner of p107 and pRb2/p130, was upregulated and underwent changes in cellular localization during differentiation. In conclusion, our data suggest a major role of pRb2/p130 in the regulation of B-myb promoter during neural differentiation despite the importance of cofactors in modulating the function of the retinoblastoma family proteins.
G Raschellà; B Tanno; F Bonetto; R Amendola; T Battista; A De Luca; A Giordano; M G Paggi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  67     ISSN:  0730-2312     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1997-12-10     Completed Date:  1997-12-10     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  297-303     Citation Subset:  IM    
Section of Toxicology and Biomedical Sciences, Ente Nuove Tecnologie Energia e Ambiente (ENEA), Rome, Italy.
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MeSH Terms
Carrier Proteins*
Cell Cycle Proteins*
Cell Differentiation
Cell Nucleus / chemistry
Cytoplasm / chemistry
DNA, Neoplasm / metabolism
DNA-Binding Proteins / analysis,  biosynthesis,  genetics*
E2F Transcription Factors
E2F4 Transcription Factor
Gene Expression Regulation, Neoplastic / genetics
Neurites / chemistry
Neuroblastoma / genetics,  metabolism*,  pathology*
Neurons / cytology*,  metabolism
Nuclear Proteins / biosynthesis,  metabolism
Phosphoproteins / biosynthesis,  metabolism*
Promoter Regions, Genetic / genetics*
Proto-Oncogene Proteins / biosynthesis
Proto-Oncogene Proteins c-myb
Retinoblastoma Protein / biosynthesis
Retinoblastoma-Binding Protein 1
Retinoblastoma-Like Protein p107
Retinoblastoma-Like Protein p130
Trans-Activators / biosynthesis
Transcription Factor DP1
Transcription Factors / analysis,  biosynthesis,  genetics*
Tumor Cells, Cultured
Grant Support
R01 CA60999-01A1/CA/NCI NIH HHS
Reg. No./Substance:
0/Carrier Proteins; 0/Cell Cycle Proteins; 0/DNA, Neoplasm; 0/DNA-Binding Proteins; 0/E2F Transcription Factors; 0/E2F4 Transcription Factor; 0/E2F4 protein, human; 0/MYBL2 protein, human; 0/Nuclear Proteins; 0/Phosphoproteins; 0/Proteins; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-myb; 0/RBL1 protein, human; 0/RBL2 protein, human; 0/Retinoblastoma Protein; 0/Retinoblastoma-Binding Protein 1; 0/Retinoblastoma-Like Protein p107; 0/Retinoblastoma-Like Protein p130; 0/Trans-Activators; 0/Transcription Factor DP1; 0/Transcription Factors

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