Document Detail


Retinoblastoma family proteins induce differentiation and regulate B-myb expression in neuroblastoma cells.
MedLine Citation:
PMID:  11464857     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The expression of several genes is modulated during neuroblastoma differentiation. The retinoblastoma family proteins, pRb, p107 and pRb2/p130, act in the repression of proliferation genes, interacting mainly with the E2F transcription factors. PROCEDURE AND RESULTS: In this study, we found that, in neuroblastoma cell lines, pRb and p107 proteins decreased, undergoing progressive dephosphorylation, whereas pRb2/p130 increased at late stages of differentiation. B-myb expression was down-regulated in association with the up-regulation of pRb2/p130, the major partner of E2F on the E2F site of the B-myb promoter in differentiated cells. Transfection of each of the retinoblastoma family genes in neuroblastoma cells was able to induce neural differentiation, to inhibit 3H-thymidine incorporation, and to down-regulate B-myb promoter activity. CONCLUSIONS: In conclusion, our data suggest a major contribution of retinoblastoma proteins, and especially of pRb2/p130, in B-myb promoter regulation and demonstrate the induction of neural differentiation by p107 and pRb2/p130, suggesting a role of these proteins in triggering differentiation-specific genes.
Authors:
G Raschellà; B Tanno; F Bonetto; A Negroni; R Amendola; M G Paggi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medical and pediatric oncology     Volume:  36     ISSN:  0098-1532     ISO Abbreviation:  Med. Pediatr. Oncol.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-07-23     Completed Date:  2001-08-16     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7506654     Medline TA:  Med Pediatr Oncol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  104-7     Citation Subset:  IM    
Affiliation:
ENEA, CR Casaccia, Section of Toxicology and Biomedical Sciences, Rome, Italy. raschella@casaccia.enea.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle Proteins*
Cell Differentiation / drug effects
DNA-Binding Proteins / biosynthesis*
Dimethyl Sulfoxide / pharmacology
E2F Transcription Factors
Gene Expression Regulation, Neoplastic*
Genes, Reporter
Genes, Retinoblastoma
Genes, myb*
Humans
Luciferases / biosynthesis
Mice
Neoplasm Proteins / genetics,  physiology*
Neuroblastoma / genetics*,  pathology
Nuclear Proteins / genetics,  physiology*
Phosphoproteins / genetics,  physiology*
Promoter Regions, Genetic
Proteins*
Recombinant Fusion Proteins / biosynthesis
Retinoblastoma Protein / physiology*
Retinoblastoma-Like Protein p107
Retinoblastoma-Like Protein p130
Trans-Activators / biosynthesis*
Transcription Factors / physiology
Transfection
Tretinoin / pharmacology
Tumor Cells, Cultured / drug effects,  metabolism
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/E2F Transcription Factors; 0/MYBL2 protein, human; 0/Mybl2 protein, mouse; 0/Neoplasm Proteins; 0/Nuclear Proteins; 0/Phosphoproteins; 0/Proteins; 0/RBL1 protein, human; 0/RBL2 protein, human; 0/Rbl1 protein, mouse; 0/Rbl2 protein, mouse; 0/Recombinant Fusion Proteins; 0/Retinoblastoma Protein; 0/Retinoblastoma-Like Protein p107; 0/Retinoblastoma-Like Protein p130; 0/Trans-Activators; 0/Transcription Factors; 302-79-4/Tretinoin; 67-68-5/Dimethyl Sulfoxide; EC 1.13.12.-/Luciferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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