Document Detail


Retinaldehyde, a potent inhibitor of gap junctional intercellular communication.
MedLine Citation:
PMID:  15500295     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Retinaldehyde and retinoic acid are derivatives of vitamin A, and retinaldehyde is the precursor for the synthesis of retinoic acid, a well-known inhibitor of gap junctional intercellular communication. In this investigation, we asked the question if retinaldehyde has similar effects on gap junctions. Gap junctional intercellular communication was measured by scrape-loading and preloading dye-transfer methods, and studies were carried out mainly on cultured liver epithelial cells. Retinaldehyde was found to be a more potent inhibitor (dye transfer reduced by 50% at 2.8 microM) than retinoic acid (dye transfer reduced by 50% at 30 microM) and glycyrrhetinic acid (dye transfer reduced by 50% at 65 microM). Both the 11-cis and all-trans forms of retinaldehyde were equally effective. Retinaldehyde inhibited dye transfer of both anionic Lucifer yellow and cationic Neurobiotin. Inhibition by retinaldehyde developed in less than two minutes at 50 microM, but unlike the reported case with retinoic acid, recovery was slower, though full. In addition to liver epithelial cells, retinaldehyde inhibited gap junctional communication in lens epithelial cells, retinal pigment epithelial cells and retinal ganglion cells.
Authors:
Sadhona Pulukuri; Ari Sitaramayya
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell communication & adhesion     Volume:  11     ISSN:  1541-9061     ISO Abbreviation:  Cell Commun. Adhes.     Publication Date:    2004 Jan-Feb
Date Detail:
Created Date:  2004-10-25     Completed Date:  2005-01-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  101096596     Medline TA:  Cell Commun Adhes     Country:  England    
Other Details:
Languages:  eng     Pagination:  25-33     Citation Subset:  IM    
Affiliation:
Eye Research Institute, Oakland University, Rochester, MI 48309-4480, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biotin / analogs & derivatives*,  antagonists & inhibitors,  physiology
Cell Adhesion Molecules / drug effects,  physiology
Cell Communication / drug effects*,  physiology
Cell Survival / drug effects,  physiology
Coloring Agents / metabolism,  pharmacokinetics
Dose-Response Relationship, Drug
Epithelial Cells / drug effects,  physiology
Gap Junctions / drug effects*,  physiology
Humans
Lens, Crystalline / cytology,  drug effects
Liver / cytology
Rats
Retinal Ganglion Cells / drug effects,  physiology
Retinaldehyde / pharmacology*
Time Factors
Grant Support
ID/Acronym/Agency:
EY 014803/EY/NEI NIH HHS; EY 07158/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Cell Adhesion Molecules; 0/Coloring Agents; 0/neurobiotin; 116-31-4/Retinaldehyde; 58-85-5/Biotin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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