Document Detail


Retinaldehyde dehydrogenase 2 is down-regulated during duodenal atresia formation in Fgfr2IIIb-/- mice.
MedLine Citation:
PMID:  21492869     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Homozygous null mutation of fibroblast growth factor receptor 2 (Fgfr2IIIb) or its ligand fibroblast growth factor 10 (Fgf10) results in duodenal atresia in mice. Mutations of either of these genes in humans cause Matthew-Wood syndrome and associated duodenal stenosis. Recently, mutations in the retinol-binding protein receptor gene STRA6 were reported to be implicated in this syndrome as well. This suggests that the retinoic acid (RA) signaling pathway interacts with the Fgf10-Fgfr2IIIb signaling pathway during duodenal development. Accordingly, we hypothesized that Fgfr2IIIb-/- mouse embryos would exhibit disruptions in expression of Raldh2, the gene for the enzyme that regulates the final step in the conversion of vitamin A to the active form RA, during duodenal atresia formation.
MATERIALS AND METHODS: Fgfr2III -/- mice were generated from heterozygous breedings. Embryos were harvested between embryonic day (E) 11.0 to E 13.5 and genotyped by polymerase chain reaction (PCR). Duodenums were dissected out, fixed and photographed. Whole mount and section in situs were performed for Raldh2.
RESULTS: Fgfr2IIIb-/- embryos demonstrate subtle changes in the duodenal morphology by E11.5 with complete involution of the atretic precursor by E 13.5. Raldh2 appears to be down-regulated as early as E 11.5 in the atretic precursor a full 2 days before this segment disappears.
CONCLUSIONS: In Fgfr2IIIb-/- mouse embryos, a reduction of Raldh2 expression is observed within the region that is forming the atresia. This is the first demonstration of such an event in this model. As in humans, these results implicate disruptions between Fgfr2IIIb receptor function and RA signaling in the formation of this defect and indicate that Fgfr2IIIb-/- mouse embryos are a valid model for the study of the atretic spectrum of defects in human duodenal development.
Authors:
Peter F Nichol; John D Tyrrell; Yukio Saijoh
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-03-21
Journal Detail:
Title:  The Journal of surgical research     Volume:  175     ISSN:  1095-8673     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-04-30     Completed Date:  2012-06-26     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  82-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Department of Surgery, Section of Pediatric Surgery, University of Wisconsin SMPH, Madison, Wisconsin 53792, USA. nichol@surgery.wisc.edu
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MeSH Terms
Descriptor/Qualifier:
Aldehyde Oxidoreductases / biosynthesis*,  genetics
Animals
Disease Models, Animal
Down-Regulation
Duodenal Obstruction / congenital,  embryology*,  genetics*
Duodenum / abnormalities,  embryology*,  metabolism*
Mice
Mice, Inbred Strains
Receptor, Fibroblast Growth Factor, Type 2 / metabolism*
Sequence Deletion
Signal Transduction / genetics
Tretinoin / metabolism
Vitamin A / metabolism
Grant Support
ID/Acronym/Agency:
1K08DK087854/DK/NIDDK NIH HHS; 1R01HD066121-01/HD/NICHD NIH HHS; K08 DK087854/DK/NIDDK NIH HHS; K08 DK087854-01/DK/NIDDK NIH HHS; K08 DK087854-03/DK/NIDDK NIH HHS; R01 HD066121/HD/NICHD NIH HHS; R01 HD066121-01/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
11103-57-4/Vitamin A; 302-79-4/Tretinoin; EC 1.2.-/Aldehyde Oxidoreductases; EC 1.2.1.36/RALDH2 protein, mouse; EC 2.7.10.1/Fgfr2 protein, mouse; EC 2.7.10.1/Receptor, Fibroblast Growth Factor, Type 2
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