| Retinal pigment epithelium rescues vascular endothelium from retinoic acid-induced apoptosis. | |
| | |
MedLine Citation:
|
PMID: 17065529 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: To determine whether retinoids are capable of inducing vascular endothelial cell apoptosis and whether the presence of an intact RPE monolayer can block retinoid-induced vascular endothelial cell death. METHODS: Confluent fetal bovine aortic endothelial (FBAE) cells were incubated with various concentrations of all-trans or 9-cis retinoic acid (an analogue of 11-cis retinoic acid). Apoptosis rates were determined at 24 hours, and the effect of inhibition of protein synthesis and activation of protein kinase C on apoptosis was investigated by supplying culture medium with 0.1 mg/mL cycloheximide and 10 nM phorbol myristate acetate. To investigate the impact of RPE on retinoid-induced apoptosis, confluent FBAE cells were cultured with a confluent layer of RPE in inserts where retinoids were added to the upper compartment. A confluent bovine corneal endothelium monolayer was used as the control. The permeabilities of the RPE and bovine corneal endothelium monolayers to fluorescein (20 microg/mL) and 9-cis retinoic acid (3 x 10(-4) M) were also determined. RESULTS: 9-cis Retinoic acid induced higher rates of apoptosis in FBAE cells than did all-trans retinoic acid and the control (P = 0.004). This effect was dose-dependent, with an ED(50) of 1.4 microM (r = 0.99, P = 0.004). Cycloheximide did not inhibit 9-cis retinoic acid-induced apoptosis, but phorbol myristate acetate significantly decreased the apoptosis rate (P = 0.005). The presence of a confluent RPE monolayer reduced the 9-cis retinoic acid-induced apoptosis rate (P = 0.002), but the presence of a bovine corneal endothelial monolayer did not (P > 0.05). Both cell types established a similar diffusion barrier against fluorescein and 9-cis retinoic acid. CONCLUSIONS: 9-cis Retinoic acid is an important mediator of vascular endothelial apoptosis. A confluent monolayer of RPE can prevent endothelial cell apoptosis, and this effect is not due simply to establishment of a diffusion barrier by the RPE. |
| | |
Authors:
|
Tongalp H Tezel; Lijun Geng; Henry J Kaplan; Lucian V Del Priore |
Related Documents
:
|
8149239 - Adult rabbit brain synthesizes retinoic acid. 8129749 - Liarozole fumarate inhibits the metabolism of 4-keto-all-trans-retinoic acid. 3702709 - Quantification of physiological levels of retinoic acid. 8757759 - Extraction of human epidermis treated with retinol yields retro-retinoids in addition t... 14621139 - Boletus manicus heim. 1333659 - Multiplicity generates diversity in the retinoic acid signalling pathways. 3564099 - Lipid metabolism, cerebral metabolic rate, and some related enzyme activities after bra... 8584419 - Peptide yy release after intraduodenal, intraileal, and intracolonic administration of ... 8219 - Enzymatic properties of native and n-ethylmaleimide-modified cardiac myosin from normal... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Investigative ophthalmology & visual science Volume: 47 ISSN: 0146-0404 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 2006 Nov |
Date Detail:
|
Created Date: 2006-10-26 Completed Date: 2006-12-08 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: United States |
Other Details:
|
Languages: eng Pagination: 5075-87 Citation Subset: IM |
Affiliation:
|
Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville School of Medicine, 301 E. Muhammad Ali Boulevard, Louisville, KY 40202, USA. tongalp.tezel@louisville.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Aged Animals Aorta, Thoracic / cytology Apoptosis / drug effects* Cattle Cell Culture Techniques Cell Survival / physiology Coculture Techniques Cycloheximide / pharmacology Dose-Response Relationship, Drug Endothelium, Vascular / cytology*, drug effects, metabolism Flow Cytometry Humans Microscopy, Fluorescence Pigment Epithelium of Eye / physiology* Protein Kinase C / metabolism Tetradecanoylphorbol Acetate / pharmacology Tretinoin / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
|
EY 016120/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
16561-29-8/Tetradecanoylphorbol Acetate; 302-79-4/Tretinoin; 5300-03-8/alitretinoin; 66-81-9/Cycloheximide; EC 2.7.11.13/Protein Kinase C |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Exploring RPE as a source of photoreceptors: differentiation and integration of transdifferentiating...
Next Document: Mapping canonical Wnt signaling in the developing and adult retina.