| Retinal pigment epithelium produces matrix metalloproteinases after laser treatment. | |
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MedLine Citation:
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PMID: 17558327 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To evaluate production of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) after panretinal photocoagulation (PRP) of human retinal pigment epithelium (RPE) explants. METHODS: Treated explants were subjected to substrate zymography to differentiate MMP-2 from MMP-9 and dot immunoblot analysis to quantify MMP-3 and TIMP activity. Tritiated thymidine uptake by RPE cells was measured to document evidence of cellular division in the laser-treated versus control explants. RESULTS: We detected MMP-2, MMP-3, and TIMP-1. MMP-2 and MMP-3 secretion increased to twice the control values. TIMP decreased until day 4 and then increased by day 6. Tritiated thymidine uptake increased 2.5-fold until day 6, returning to baseline by day 8. CONCLUSION: PRP disturbs MMP/TIMP balance, inhibiting the initiation and maintenance required for active neovascularization. The efficacy of PRP may be due to changes in the expression pattern of metalloproteinases and inhibitors. This model elucidates the possible contribution of PRP to neovascularization regression by demonstrating the effect of laser on TIMP/MMP balance. The effects of PRP may be much more complex than currently understood and most likely involve more than vascular endothelial growth factor and other ischemia-related factors. |
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Authors:
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Christina Flaxel; John Bradle; Ted Acott; John R Samples |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Retina (Philadelphia, Pa.) Volume: 27 ISSN: 0275-004X ISO Abbreviation: Retina (Philadelphia, Pa.) Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-06-11 Completed Date: 2007-07-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8309919 Medline TA: Retina Country: United States |
Other Details:
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Languages: eng Pagination: 629-34 Citation Subset: IM |
Affiliation:
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Glaucoma Service, Casey Eye Institute, Oregon Health & Science University, 3375 SW Terwilliger Boulevard, Portland, OR 97239-4197, USA. flaxelc@ohsu.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Blotting, Western Cell Division Electrophoresis, Polyacrylamide Gel Humans Laser Coagulation* Matrix Metalloproteinases / biosynthesis* Middle Aged Organ Culture Techniques Pigment Epithelium of Eye / enzymology*, surgery* Tissue Inhibitor of Metalloproteinases / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/Tissue Inhibitor of Metalloproteinases; EC 3.4.24.-/Matrix Metalloproteinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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