| Retinal heat shock protein 25 in early experimental diabetes. | |
| | |
MedLine Citation:
|
PMID: 22068623 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Diabetic retinopathy is the leading cause of blindness in adults, and oxidative stress has been pathogenically associated with retinal neurodegeneration. Cellular stresses induce expression of heat shock proteins (HSPs) and this results in cytoprotection. Our aim was to assess retinal expression of HSP25 in early experimental diabetes. Mice were rendered diabetic by streptozotocin injection. Ten weeks after diabetes onset retinal HSP25 expression were studied by real-time PCR, immunoblotting and immunohistochemistry (IHC). Expression of nitrotyrosine and Cu/Zn superoxide dismutase (SOD), was assessed by IHC and apoptosis by TUNEL. Retinal HSP25 mRNA and protein expression was significantly increased in diabetic as compared to non-diabetic animals and localised predominantly within the retinal ganglion cells (RGC) layer. This was paralleled overexpression of nitrotyrosine and SOD and enhanced apoptosis. In early experimental diabetes, HSP25 is overexpressed in the RGC layer in parallel with markers of oxidative stress and apoptosis. |
| | |
Authors:
|
Silvia Pinach; Davina Burt; Elena Berrone; Federica Barutta; Graziella Bruno; Massimo Porta; Paolo Cavallo Perin; Gabriella Gruden |
Related Documents
:
|
21855553 - Rat liver uncoupling protein 2: changes induced by a fructose-rich diet. 21153133 - Reduced hepatic sterol carrier protein-2 expression in the streptozotocin treated diabe... 6219183 - Influence of streptozotocin-induced diabetes in pregnant rats on plasma corticosterone ... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-11-9 |
Journal Detail:
|
Title: Acta diabetologica Volume: - ISSN: 1432-5233 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-11-9 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9200299 Medline TA: Acta Diabetol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Internal Medicine, University of Turin, C/so Dogliotti 14, 10126, Turin, Italy, silvia.pinach@unito.it. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Obesity is associated with lower mortality risk in elderly diabetic subjects: the Casale Monferrato ...
Next Document: The collaborative role of molecular conformation and energetics in the binding of gas-phase non-cova...