| Retinal ganglion cells recognized by serum autoantibody against gamma-enolase found in glaucoma patients. | |
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MedLine Citation:
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PMID: 10845582 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To study pathologic roles of the presence of serum autoantibodies against retinal ganglion cells in patients with glaucoma. METHODS: Serum autoantibody reactions were detected by Western blot analysis using retinal soluble fractions in 79 patients with glaucoma (normal-tension glaucoma [NTG], 23 cases; primary open-angle glaucoma [POAG], 56 cases) and 60 age-matched healthy subjects. Clinical characteristics including visual acuity, visual field, intraocular pressure (IOP), and optic disc features were compared between the serum autoantibody-positive and -negative patients. The retinal autoantigen recognized by patients' sera was identified by a combination of in-gel digestion and Edman sequencing. RESULTS: Western blot analysis revealed that serum autoantibody against retinal 50-kDa antigen was recognized in 20 out of 79 glaucoma patients (25.3%; 14 POAG and 6 NTG patients) and 60 age-matched control subjects (11.7%), respectively. Immunocytochemistry revealed that labeling of the ganglion cell layer (GCL) by IgG from glaucoma patients (POAG: 13/56, 23.2%; NTG: 6/23, 26%) existed at a significantly higher rate than that by IgG from control subjects (2/60, 3.3%; P < 0.05). In POAG, maximum IOP in the serum antibody positive-patients was significantly lower than that in the antibody-negative patients (P < 0.05). However, no statistical differences were observed in visual field loss, disc cupping, and other clinical factors between the antibody-positive and -negative groups in POAG and NTG. In-gel digestion of the 50-kDa band in two-dimensional polyacrylamide gels and Edman sequence analysis of the high-performance liquid chromatography-purified peptides identified the 50-kDa protein as gamma-enolase. Injection of the 50-kDa IgG from glaucoma patients or anti-gamma-enolase serum into the vitreous cavity of Lewis rats caused reduction of the b-wave of the electroretinogram and TdT-dUTP terminal nick-end labeling (TUNEL)-positive staining within the GCL. CONCLUSIONS: In the current study, serum autoantibody against 50-kDa protein identified as gamma-enolase in 25% of glaucoma patients. |
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Authors:
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I Maruyama; H Ohguro; Y Ikeda |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 41 ISSN: 0146-0404 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 2000 Jun |
Date Detail:
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Created Date: 2000-06-14 Completed Date: 2000-06-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1657-65 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, Sapporo Medical University School of Medicine, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Amino Acid Sequence Animals Autoantibodies / blood* Autoantigens / immunology* Blotting, Western Chromatography, High Pressure Liquid Electrophoresis, Gel, Two-Dimensional Electroretinography Female Fluorescent Antibody Technique, Indirect Glaucoma, Open-Angle / immunology*, pathology Humans Immunoglobulin G / analysis Intraocular Pressure Male Middle Aged Molecular Sequence Data Molecular Weight Ocular Hypertension / immunology, pathology Phosphopyruvate Hydratase / immunology* Rats Rats, Inbred Lew Retinal Ganglion Cells / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Autoantibodies; 0/Autoantigens; 0/Immunoglobulin G; EC 4.2.1.11/Phosphopyruvate Hydratase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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