Document Detail


Reticulocyte quantification by Coulter MAXM VCS (volume, conductivity, light scatter) technology.
MedLine Citation:
PMID:  8969628     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study the ability of the Coulter MAXM analyzer to quantify reticulocytes was evaluated. The results were compared with those obtained by a microscopic method according to NCCLS H44-P recommendations and with the results from the automated analyzer Sysmex R-1000. Duplicate samples from 330 patients were analyzed. The reference intervals obtained with the three methods were: MAXM 0.37-1.80%, median 0.83%; manual 0.40-2.30%, median 1.00%; R-1000 0.60-1.95%, median 1.06%. The imprecision (CV) at all concentrations is lower than the microscopic method (low 16.1% vs 67%; normal 16.9% vs 28.9%; high 9.5% vs 13.0%). The MAXM shows a good overall correlation with the microscopic method (intercept 0.01, slope 0.89, r2 = 0.87) despite evidence of a significant overestimation at low concentrations [difference (D) 0.30] and a moderate underestimation at normal (D -0.15) and high (D -1.04) concentrations; the same behavior is shown in comparison with results from the R-1000, with an overall underestimation by MAXM (D -0.26). When compared with the microscopic method, MAXM shows a modest sensitivity at low reticulocyte counts (54.8%) and satisfactory sensitivity for high counts (87.3%), with an overall agreement of 81.3%.
Authors:
M Buttarello; P Bulian; M D Prà; P Barbera; P Rizzotti
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  42     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-01-17     Completed Date:  1997-01-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1930-7     Citation Subset:  IM    
Affiliation:
Laboratorio Analisi Chimico-Cliniche e Microbiologiche, Ospedale Geriatrico, Padova, Italia.
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MeSH Terms
Descriptor/Qualifier:
Autoanalysis
Humans
Microscopy
Quality Control
Reference Values
Regression Analysis
Reticulocyte Count / instrumentation*,  methods
Sensitivity and Specificity

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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