Document Detail

Reticulocyte lipoxygenase exhibits both n-6 and n-9 activities.
MedLine Citation:
PMID:  6413249     Owner:  NLM     Status:  MEDLINE    
Purified reticulocyte lipoxygenase converts arachidonic acid to both 15- and 12-hydroxyperoxyeico-satetraenoic acids. The proportion of the two reaction products does not change during the purification procedure as shown by HPLC analysis. By means of isoelectric focusing it was not possible to separate the n-6 and n-9 activities. Reticulocyte lipoxygenase was completely inactivated by both 5,8,11-eicosatriynoic and 5,8,11,14-eicosatetraynoic acids in contrast to soybean lipoxygenase-1 which was inactivated only by 5,8,11,14-eicosatetraynoic acid. These results indicate that reticulocyte lipoxygenase exhibits both n-6 and n-9 activities. A contamination of the enzyme preparation with other lipoxygenases, e.g., the n-9 lipoxygenase from thrombocytes appears to be excluded.
H Kühn; R Wiesner; T Schewe; S M Rapoport
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  153     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1983 Mar 
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  353-6     Citation Subset:  IM    
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MeSH Terms
Chromatography, High Pressure Liquid
Lipoxygenase / blood*,  isolation & purification
Plants / enzymology
Reticulocytes / enzymology*
Species Specificity
Substrate Specificity
Reg. No./Substance:

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