| Reticulocyte count in red-blood-cell units stored in AS-1. | |
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MedLine Citation:
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PMID: 23347270 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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BACKGROUND AND OBJECTIVES: Previous data that showed maintenance of reticulocyte percentage in whole blood stored in CPDA-1 have led to the assumption that reticulocyte maturation becomes arrested during refrigerated storage. However, reticulocyte behaviour in red-blood-cell units stored in additive solutions has not yet been studied. This study was thus aimed at determining reticulocyte count and reticulocyte subtypes in red-blood-cells units stored in AS-1. MATERIALS AND METHODS: Reticulocyte percentage and subtypes were determined by flow cytometry with thiazole orange in six red-blood-cells units stored in AS-1. RESULTS: Reticulocyte count was 26·8 ± 4·6 × 10(9) /l at week 0·5 and 8·2 ±2·9 × 10(9) /l at week 6. Total haemolysis during storage was 0·19 ± 0·08%. High-fluorescence reticulocytes were 2·0 ± 3·2 × 10(9) /l at week 0·5 and decreased by weeks 2, 4 and 6. Low-fluorescence reticulocytes were 22·1 ± 3·1 × 10(9) /l at week 0·5 and decreased by weeks 4 and 6. CONCLUSION: A significant decrease in reticulocytes occurred during red-blood-cells units' storage in AS-1. Even if it were assumed that all of haemolysed cells during storage were reticulocytes, there are a number of them whose disappearance cannot be explained by this mechanism. Changes observed in reticulocyte subtypes suggest that they mature during storage. |
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Authors:
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A Urbina; F Palomino |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-25 |
Journal Detail:
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Title: Vox sanguinis Volume: - ISSN: 1423-0410 ISO Abbreviation: Vox Sang. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0413606 Medline TA: Vox Sang Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2013 The Author(s) Vox Sanguinis © 2013 International Society of Blood Transfusion. |
Affiliation:
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School of Medicine and Health Sciences, Basic Science Department, Physiology Unit, Universidad del Rosario, Bogotá, DC, Colombia. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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