Document Detail

Rethinking synchronization of mammalian cells for cell cycle analysis.
MedLine Citation:
PMID:  12861378     Owner:  NLM     Status:  MEDLINE    
An analysis of different classes of forced or batch synchronization methods reveals why these methods, in theory, do not produce synchronized cultures. Cells may be aligned for a particular property after specific treatments, but these aligned cells do not correspond to any particular cell age during the normal cell cycle. The experimental methods analyzed are those that arrest cells with a G1 phase amount of DNA, those that inhibit DNA synthesis, and those that arrest cells at mitosis. Release of arrested cells from inhibition does not produce cells reflecting cells during the normal division cycle. Thus, cells produced by batch or forcing methods are not experimental models for analysis of the normal cell cycle.
S Cooper
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Cellular and molecular life sciences : CMLS     Volume:  60     ISSN:  1420-682X     ISO Abbreviation:  Cell. Mol. Life Sci.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-07-15     Completed Date:  2003-08-05     Revised Date:  2005-11-16    
Medline Journal Info:
Nlm Unique ID:  9705402     Medline TA:  Cell Mol Life Sci     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  1099-106     Citation Subset:  IM    
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109-0620, USA.
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MeSH Terms
Cell Culture Techniques / methods*
Cell Cycle*
Cell Division
DNA / biosynthesis
G1 Phase
Mitosis / drug effects
Models, Biological
Nocodazole / pharmacology
Reg. No./Substance:
31430-18-9/Nocodazole; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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