Document Detail

Retention of spliceosomal components along ligated exons ensures efficient removal of multiple introns.
MedLine Citation:
PMID:  20610656     Owner:  NLM     Status:  MEDLINE    
The majority of mammalian pre-mRNAs contains multiple introns that are excised prior to export and translation. After intron excision, ligated exon intermediates participate in subsequent intron excisions. However, exon ligation generates an exon of increased size, a feature of pre-mRNA splicing that can interfere with downstream splicing events. These considerations raise the question of whether unique mechanisms exist that permit efficient removal of introns neighboring ligated exons. Kinetic analyses of multiple intron-containing pre-mRNAs revealed that splicing is more efficient following an initial intron removal event, suggesting that either the recruitment of the exon junction complex (EJC) to ligated exons increases the efficiency of multiple intron excisions or that the initial definition of splice sites is sufficient to permit efficient splicing of introns neighboring ligated exons. Knockdown experiments show that the deposition of the EJC does not affect subsequent splicing kinetics. Instead, spliceosomal components that are not involved in the initial splicing event remain associated with the pre-mRNA to ensure efficient removal of neighboring introns. Thus, ligated exons do not require redefinition, providing an additional kinetic advantage for exon defined splice sites.
Tara L Crabb; Bianca J Lam; Klemens J Hertel
Related Documents :
11322776 - A point mutation in a cadherin gene, cdh23, causes deafness in a novel mutant, waltzer ...
14645916 - Complex alternative processing of human cytomegalovirus ul37 pre-mrna.
7585246 - Sl1 trans-splicing specified by au-rich synthetic rna inserted at the 5' end of caenorh...
8915536 - Function of a pseudoknot in the suppression of an alternative splicing event in a group...
18587586 - Investigation of fgf10 as a candidate gene in patients with anorectal malformations and...
2987246 - A strong sequence homology exists between the major rna polymerase sigma factors of bac...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-07-07
Journal Detail:
Title:  RNA (New York, N.Y.)     Volume:  16     ISSN:  1469-9001     ISO Abbreviation:  RNA     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-18     Completed Date:  2010-09-21     Revised Date:  2013-12-11    
Medline Journal Info:
Nlm Unique ID:  9509184     Medline TA:  RNA     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1786-96     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Drosophila / genetics
Drosophila Proteins / genetics
Gene Knockdown Techniques
HeLa Cells
Nerve Tissue Proteins / genetics
RNA Splicing*
Spliceosomes / metabolism*
Transcription Factors / genetics
beta-Globins / genetics
Grant Support
Reg. No./Substance:
0/Drosophila Proteins; 0/Nerve Tissue Proteins; 0/Transcription Factors; 0/beta-Globins; 0/fru protein, Drosophila

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The Mannose Receptor Mediates the Uptake of Diverse Native Allergens by Dendritic Cells and Determin...
Next Document:  FIP1/RCP binding to Golgin-97 regulates retrograde transport from recycling endosomes to the trans-G...