Document Detail


Retention of metabolites of 2',3'-didehydro-3'-deoxy-4'-ethynylthymidine, a novel anti-human immunodeficiency virus type 1 thymidine analog, in cells.
MedLine Citation:
PMID:  19470503     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
2',3'-Didehydro-3'-deoxy-4'-ethynylthymidine (4'-Ed4T), a novel thymidine analog, has more potent anti-human immunodeficiency virus type 1 (HIV-1) activity than its progenitor, stavudine (d4T). The profile of the intracellular metabolites of 4'-Ed4T was qualitatively similar to that of zidovudine (AZT) but not to that of d4T, while after drug removal it showed more persistent anti-HIV activity than AZT or d4T in cell culture. When CEM cells were exposed to various concentrations of 4'-Ed4T, 4'-Ed4T was efficiently taken up by the cells and was readily phosphorylated to 4'-Ed4T monophosphate (4'-Ed4TMP), 4'-Ed4T diphosphate (4'-Ed4TDP), and 4'-Ed4T triphosphate (4'-Ed4TTP). Most importantly, 4'-Ed4TTP, the active metabolite of 4'-Ed4T, persisted significantly longer than 4'-Ed4TDP and 4'-Ed4TMP after drug removal. We further investigated the efflux profiles of 4'-Ed4T in the comparison with those of AZT in CEM cells. After drug removal, both 4'-Ed4T and AZT were effluxed from the cells in a time- and temperature-dependent manner. However, the efflux of 4'-Ed4T from cells was much less efficient than that of AZT. 4'-Ed4T was effluxed from cells only in its nucleoside form, while AZT was effluxed from cells in both its nucleoside and monophosphate forms. The mechanism-of-action study showed that the efflux of 4'-Ed4T or AZT nucleoside might be due to unknown nucleoside transporters which were not related to the equilibrative nucleoside transporters, while the efflux of AZT monophosphate might be due to multidrug resistance protein 4 (MRP4/ABCC4). The results demonstrated that no detectable 4'-Ed4TMP efflux and the less efficient efflux of 4'-Ed4T nucleoside from cells might be one of the biochemical determinants of its persistent antiviral activity in cell culture.
Authors:
Xin Wang; Hiromichi Tanaka; Masanori Baba; Yung-chi Cheng
Related Documents :
7509193 - Interaction of multidrug-resistant chinese hamster ovary cells with the peptide ionopho...
9096663 - Annamycin circumvents resistance mediated by the multidrug resistance-associated protei...
24777333 - Antitumor activity of a polysaccharide from longan seed on lung cancer cell line a549 i...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-05-26
Journal Detail:
Title:  Antimicrobial agents and chemotherapy     Volume:  53     ISSN:  1098-6596     ISO Abbreviation:  Antimicrob. Agents Chemother.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-20     Completed Date:  2009-10-19     Revised Date:  2010-09-27    
Medline Journal Info:
Nlm Unique ID:  0315061     Medline TA:  Antimicrob Agents Chemother     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3317-24     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, SHM B226, New Haven, CT 06520, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Anti-HIV Agents / metabolism*
Biological Transport / drug effects,  genetics
Cell Line
Cell Line, Tumor
Chromatography, High Pressure Liquid
Dipyridamole / pharmacology
Humans
Multidrug Resistance-Associated Proteins / genetics,  physiology
Phosphodiesterase Inhibitors / pharmacology
Phosphorylation
Stavudine / analogs & derivatives*,  metabolism
Thymidine / analogs & derivatives*
Zidovudine / metabolism
Grant Support
ID/Acronym/Agency:
AI-38204/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/4'-ethynylstavudine; 0/ABCC4 protein, human; 0/Anti-HIV Agents; 0/Multidrug Resistance-Associated Proteins; 0/Phosphodiesterase Inhibitors; 30516-87-1/Zidovudine; 3056-17-5/Stavudine; 50-89-5/Thymidine; 58-32-2/Dipyridamole
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The role of thoracoscopy for the diagnosis of hidden diaphragmatic injuries in penetrating thoracoab...
Next Document:  Outer membrane protein STM3031 (Ail/OmpX-like protein) plays a key role in the ceftriaxone resistanc...