| Resveratrol suppresses apoptosis in intact human cardiac tissue - In vitro model simulating extracorporeal circulation. | |
| | |
MedLine Citation:
|
PMID: 21577194 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
AIM: After cardioplegia and subsequent reperfusion of the myocardium as employed in cardiac surgery, ischemia/reperfusion injury of the myocardium can induce apoptosis. The aim of this study was to evaluate the anti-apoptotic properties of resveratrol, a phenolic phytoalexin present in grape skins and especially red wines during simulated cardioplegia (cp) and reperfusion (rep) in an in-vitro microperfusion model on human myocardium, which to our knowledge has not been investigated yet. METHODS: Cardiac specimens were retrieved from the right auricle of patients undergoing elective coronary artery bypass graft before induction of cardiopulmonary bypass. Cardiac specimens, with resveratrol (10 µM) (N.=15) and w/o resveratrol (control, N.=15) were exposed in vitro to varying periods of cp/rep (30/10, 60/20, 120/40 min) in a microperfusion chamber. For detection of apoptosis anti-activated-caspase-3, PARP-1 cleavage immunostaining and real-time PCR for gene expression of cardiac cytokines like BNP, NF-κB1, NF-κB2, E-Selectin, Troponin and TNF-α were employed. RESULTS: Control group: the longer the cp/rep period lasted the higher were the rate of anti-activated-caspase-3 positive cardiomyocytes (21.26±2.07% ‑ 46.56±3.2%) and of PARP1-cleavage positive cardiomyocytes (23.29±2.16% ‑ 36.86±2.11%). Resveratrol group: apoptosis was suppressed significantly (P<0.05). Anti-activated-caspase-3 positive cardiomyocytes (13.45±4.35% ‑ 15.3±2.97%) and PARP1-cleavage positive cardiomyocytes (9.87±2.04% ‑ 11.77±3.42%). Resveratrol significantly suppressed the expression of BNP, NF-κB2, E-Selectin, Troponin and TNF-α in vitro (P<0.05). CONCLUSION: Resveratrol significantly suppresses apoptosis under our applied in vitro conditions. This finding warrants further studies aiming suppression of ischemia/reperfusion injury in clinical settings. |
| | |
Authors:
|
E Usta; M Mustafi; T Walker; G Ziemer |
Related Documents
:
|
16330064 - Peroxisome-proliferator-activated receptor-gamma agonists inhibit the release of proinf... 19128264 - Anti-apoptotic role of egf in hacat keratinocytes via a pparbeta-dependent mechanism. 11223164 - Agonists of the retinoic acid- and retinoid x-receptors inhibit hepatocyte growth facto... 21651924 - Simvastatin induces apoptosis by a rho-dependent mechanism in cultured cardiac fibrobla... 17196594 - Conservation of cd4+ t cell-dependent developmental mechanisms in the blood fluke patho... 10049664 - Inhibition of microvascular endothelial apoptosis in tissue explants by serum albumin. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The Journal of cardiovascular surgery Volume: 52 ISSN: 0021-9509 ISO Abbreviation: J Cardiovasc Surg (Torino) Publication Date: 2011 Jun |
Date Detail:
|
Created Date: 2011-05-17 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0066127 Medline TA: J Cardiovasc Surg (Torino) Country: Italy |
Other Details:
|
Languages: eng Pagination: 399-409 Citation Subset: IM |
Affiliation:
|
Department of Cardiac and Vascular Surgery, University Hospital Tübingen, Tübingen, Germany - engin.usta@gmx.de. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Influence of surgical or endovascular distal revascularization of the lower limbs on ischemic ulcer ...
Next Document: The acute effect of IABP-induced pulsatility on coronary vascular resistance and graft flow in criti...