Document Detail


Resveratrol, purified from the stem of Vitis coignetiae Pulliat, inhibits food intake in C57BL/6J Mice.
MedLine Citation:
PMID:  20512477     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neuropeptide Y (NPY) and agouti-related protein (AgRP) have powerful stimulatory effects on food intake, which suggests that the downregulation of brain NPY or AgRP may help reduce obesity and diabetes by inhibiting food intake. To search for active compounds that inhibit NPY and AgRP expression, we made two luciferase reporter assay systems consisting of NPY and AgRP promoter-driven luciferase genes, together with the puromycin resistance gene, in a plasmid vector. Each plasmid was permanently transfected into N29-4 neuronal cells. Using the systems, resveratrol was purified from the stem of Vitis coignetiae Pulliat by activityguided fractionation. Resveratrol downregulated NPY and AgRP promoter-driven luciferase activity in a dose-dependent manner. The inhibitory concentrations (IC(50), 50% inhibition) of resveratrol against pNPY-luc and pAgRP-luc activities were 8.9 microM and 8.0 microM, respectively. Furthermore, one-time intraperitoneal injection of resveratrol (100 mg/kg) suppressed 20.0% and 17.2% of food intake during 24 and 48 h, respectively. These results indicated that resveratrol inhibited food intake, which may be related to the downregulation of NPY and AgRP gene expression.
Authors:
Su-Jin Kim; Yong Hwa Lee; Man-Deuk Han; Woongchon Mar; Won-Ki Kim; Kung-Woo Nam
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-29
Journal Detail:
Title:  Archives of pharmacal research     Volume:  33     ISSN:  0253-6269     ISO Abbreviation:  Arch. Pharm. Res.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-31     Completed Date:  2010-09-16     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  8000036     Medline TA:  Arch Pharm Res     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  775-80     Citation Subset:  IM    
Affiliation:
Jung San Bio Technology, Hwaseong, Korea.
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MeSH Terms
Descriptor/Qualifier:
Agouti-Related Protein / genetics,  metabolism
Animals
Anti-Obesity Agents / isolation & purification*,  pharmacology*
Cell Line
Cell Survival / drug effects
Dose-Response Relationship, Drug
Down-Regulation / drug effects
Eating / drug effects*
Hep G2 Cells
Humans
Male
Mice
Mice, Inbred C57BL
Neurons / metabolism
Neuropeptide Y / genetics,  metabolism
Plant Stems / chemistry
Random Allocation
Stilbenes / isolation & purification*,  pharmacology*
Vitis / chemistry*
Chemical
Reg. No./Substance:
0/Agouti-Related Protein; 0/Anti-Obesity Agents; 0/Neuropeptide Y; 0/Stilbenes; Q369O8926L/resveratrol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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