| Resveratrol, purified from the stem of Vitis coignetiae Pulliat, inhibits food intake in C57BL/6J Mice. | |
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MedLine Citation:
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PMID: 20512477 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Neuropeptide Y (NPY) and agouti-related protein (AgRP) have powerful stimulatory effects on food intake, which suggests that the downregulation of brain NPY or AgRP may help reduce obesity and diabetes by inhibiting food intake. To search for active compounds that inhibit NPY and AgRP expression, we made two luciferase reporter assay systems consisting of NPY and AgRP promoter-driven luciferase genes, together with the puromycin resistance gene, in a plasmid vector. Each plasmid was permanently transfected into N29-4 neuronal cells. Using the systems, resveratrol was purified from the stem of Vitis coignetiae Pulliat by activityguided fractionation. Resveratrol downregulated NPY and AgRP promoter-driven luciferase activity in a dose-dependent manner. The inhibitory concentrations (IC(50), 50% inhibition) of resveratrol against pNPY-luc and pAgRP-luc activities were 8.9 microM and 8.0 microM, respectively. Furthermore, one-time intraperitoneal injection of resveratrol (100 mg/kg) suppressed 20.0% and 17.2% of food intake during 24 and 48 h, respectively. These results indicated that resveratrol inhibited food intake, which may be related to the downregulation of NPY and AgRP gene expression. |
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Authors:
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Su-Jin Kim; Yong Hwa Lee; Man-Deuk Han; Woongchon Mar; Won-Ki Kim; Kung-Woo Nam |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-29 |
Journal Detail:
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Title: Archives of pharmacal research Volume: 33 ISSN: 0253-6269 ISO Abbreviation: Arch. Pharm. Res. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-31 Completed Date: 2010-09-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8000036 Medline TA: Arch Pharm Res Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 775-80 Citation Subset: IM |
Affiliation:
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Jung San Bio Technology, Hwaseong, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Agouti-Related Protein
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genetics,
metabolism Animals Anti-Obesity Agents / isolation & purification*, pharmacology* Cell Line Cell Survival / drug effects Dose-Response Relationship, Drug Down-Regulation / drug effects Eating / drug effects* Hep G2 Cells Humans Male Mice Mice, Inbred C57BL Neurons / metabolism Neuropeptide Y / genetics, metabolism Plant Stems / chemistry Random Allocation Stilbenes / isolation & purification*, pharmacology* Vitis / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Agouti-Related Protein; 0/Anti-Obesity Agents; 0/Neuropeptide Y; 0/Stilbenes; 501-36-0/resveratrol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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