| Resveratrol protects primary rat hepatocytes against oxidative stress damage: activation of the Nrf2 transcription factor and augmented activities of antioxidant enzymes. | |
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MedLine Citation:
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PMID: 18616940 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oxidative stress is recognized as an important factor in the development of liver pathologies. The reactive oxygen species endogenously generated or as a consequence of xenobiotic metabolism are eliminated by enzymatic and nonenzymatic cellular systems. Besides endogen defences, the antioxidant consumption in the diet has an important role in the protection against the development of diseases product of oxidative damage. Resveratrol is a naturally occurring compound which is part of the human diet. This molecule has been shown to have many biological properties, including antioxidant activity. We decided to test if resveratrol could protect primary hepatocytes in culture from oxidative stress damage and if so, to determine if this compound affects the cellular detoxifying systems and their regulation through the Nrf2 transcription factor that regulates the expression of antioxidant and phase II detoxifying enzymes. Cell death by necrosis was detected by measuring the activity of lactate dehydrogenase liberated to the medium. The activities of antioxidant and phase II enzymes were measured using previously described methods. Activation of the Nrf2 transcription factor was studied by confocal microscopy and the Nrf2 and its coding mRNA levels were determined by western blot and quantitative PCR respectively. Resveratrol pre-treatment effectively protected hepatocytes in culture exposed to oxidative stress, increasing the activities of catalase, superoxide dismutase, glutathione peroxidase, NADPH quinone oxidoreductase and glutathione-S-transferase. Resveratrol increases the level of Nrf2 and induces its translocation to the nucleus. Also, it increases the concentration of the coding mRNA for Nrf2. In this work we show that resveratrol could be a useful drug for the protection of liver cells from oxidative stress induced damage. |
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Authors:
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Juan Andrés Rubiolo; Gilles Mithieux; Félix Victor Vega |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-06-22 |
Journal Detail:
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Title: European journal of pharmacology Volume: 591 ISSN: 0014-2999 ISO Abbreviation: Eur. J. Pharmacol. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-08-12 Completed Date: 2008-10-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1254354 Medline TA: Eur J Pharmacol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 66-72 Citation Subset: IM |
Affiliation:
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Departamento de Fisiología, Facultad de Veterinaria Universidad de Santiago de Compostela, 27002, Lugo, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antioxidants / metabolism, pharmacology* Blotting, Western Cells, Cultured Enzymes / drug effects, metabolism Gene Expression Regulation / drug effects Hepatocytes / drug effects, metabolism Male NF-E2-Related Factor 2 / drug effects*, metabolism Necrosis / etiology Oxidative Stress / drug effects* Polymerase Chain Reaction RNA, Messenger / drug effects, metabolism Rats Rats, Sprague-Dawley Stilbenes / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Enzymes; 0/NF-E2-Related Factor 2; 0/RNA, Messenger; 0/Stilbenes; 501-36-0/resveratrol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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