| Resveratrol, a phytoestrogen found in red wine, down-regulates protein S expression in HepG2 cells. | |
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MedLine Citation:
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PMID: 20926118 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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MATERIALS AND METHODS: The secreted and intracellular levels of PS were determined by an enzyme-linked ligandsorbent assay and Western blotting. The mRNA expressions of PS, PC and β chain of C4b-binding protein (C4BP-β) were analyzed by reverse transcription-polymerase chain reaction. The PS gene promotor activities in HepG2 cells transiently expressing estrogen receptor (ER) α were examined by a luciferase reporter assay. RESULTS: Resveratrol dose- and time-dependently down-regulated the PS expression in HepG2 cells at a transcriptional level, resulting in a significant decrease in secreted PS; however, the PC and C4BP-β mRNA expressions were not affected. This action of resveratrol was not mediated through either the ER signaling or those of mitogen-activated protein kinases and protein kinase C. Piceatannol, a hydroxylated metabolite of resveratrol, and genistein, an isoflavone found in soy products, also down-regulated the PS expression. CONCLUSIONS: Resveratrol down-regulates the PS expression in HepG2 cells in an ER-independent manner, and the two phenolic hydroxyls at carbon-3 and -5 of resveratrol may be involved in this function. |
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Authors:
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Yoshie Hiroto; Kana Tadokoro; Tomohide Tsuda; Eri Nakazono; Keizo Ohnaka; Ryoichi Takayanagi; Naotaka Hamasaki; Hiroko Tsuda |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Thrombosis research Volume: 127 ISSN: 1879-2472 ISO Abbreviation: Thromb. Res. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0326377 Medline TA: Thromb Res Country: United States |
Other Details:
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Languages: eng Pagination: e1-7 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Graduate School of Health and Nutrition Sciences, Nakamura Gakuen University, Fukuoka, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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