Document Detail

Resveratrol, a phytoestrogen found in red wine, down-regulates protein S expression in HepG2 cells.
MedLine Citation:
PMID:  20926118     Owner:  NLM     Status:  MEDLINE    
MATERIALS AND METHODS: The secreted and intracellular levels of PS were determined by an enzyme-linked ligandsorbent assay and Western blotting. The mRNA expressions of PS, PC and β chain of C4b-binding protein (C4BP-β) were analyzed by reverse transcription-polymerase chain reaction. The PS gene promotor activities in HepG2 cells transiently expressing estrogen receptor (ER) α were examined by a luciferase reporter assay.
RESULTS: Resveratrol dose- and time-dependently down-regulated the PS expression in HepG2 cells at a transcriptional level, resulting in a significant decrease in secreted PS; however, the PC and C4BP-β mRNA expressions were not affected. This action of resveratrol was not mediated through either the ER signaling or those of mitogen-activated protein kinases and protein kinase C. Piceatannol, a hydroxylated metabolite of resveratrol, and genistein, an isoflavone found in soy products, also down-regulated the PS expression.
CONCLUSIONS: Resveratrol down-regulates the PS expression in HepG2 cells in an ER-independent manner, and the two phenolic hydroxyls at carbon-3 and -5 of resveratrol may be involved in this function.
Yoshie Hiroto; Kana Tadokoro; Tomohide Tsuda; Eri Nakazono; Keizo Ohnaka; Ryoichi Takayanagi; Naotaka Hamasaki; Hiroko Tsuda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Thrombosis research     Volume:  127     ISSN:  1879-2472     ISO Abbreviation:  Thromb. Res.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-24     Completed Date:  2011-04-08     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  0326377     Medline TA:  Thromb Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e1-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Graduate School of Health and Nutrition Sciences, Nakamura Gakuen University, Fukuoka, Japan.
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MeSH Terms
Blood Proteins / genetics,  metabolism*
Blotting, Western
Carcinoma, Hepatocellular / genetics,  metabolism*
Dose-Response Relationship, Drug
Enzyme-Linked Immunosorbent Assay
Estrogen Receptor alpha / metabolism
Gene Expression Regulation, Neoplastic / drug effects
Genistein / pharmacology
Hep G2 Cells
Histocompatibility Antigens / metabolism
Liver Neoplasms / genetics,  metabolism*
Molecular Structure
Phytoestrogens / chemistry,  pharmacology*
Promoter Regions, Genetic / drug effects
Protein C / metabolism
Protein S / genetics,  metabolism*
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Stilbenes / chemistry,  pharmacology*
Structure-Activity Relationship
Time Factors
Transcription, Genetic / drug effects
Reg. No./Substance:
0/Blood Proteins; 0/C4BPB protein, human; 0/Estrogen Receptor alpha; 0/Histocompatibility Antigens; 0/PROS1 protein, human; 0/Phytoestrogens; 0/Protein C; 0/Protein S; 0/RNA, Messenger; 0/Stilbenes; 4339-71-3/3,3',4,5'-tetrahydroxystilbene; 446-72-0/Genistein; Q369O8926L/resveratrol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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