| Resveratrol-mediated apoptosis of hodgkin lymphoma cells involves SIRT1 inhibition and FOXO3a hyperacetylation. | |
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MedLine Citation:
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PMID: 22833338 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Resveratrol (RSV), a plant-derived stilbene, induces cell death in Hodgkin lymphoma (HL)-derived L-428 cells in a dose-dependent manner (IC50=27 μM, trypan blue exclusion assay). At a lower range (25 μM), RSV treatment for 48 hours causes arrest in the S-phase of the cell cycle, while at a higher concentration range (50 μM), apoptosis can be detected, with activation of caspase-3. The histone/protein deacetylase SIRT1 has been described as a putative target of RSV action in other model systems, even though its role in cancer cells is still controversial. Here we show that RSV, at both concentration ranges, leads to a marked increase in p53, while a decrease of SIRT1 expression level, as well as enzyme activity, only occurred at the higher concentration range. Concomitantly, however, treatments at both concentration ranges resulted in a marked increase in K373-acetylated p53 and lysine-acetylated FOXO3a. Immunohistochemical stainings of human lymph nodes show a preferential distribution of SIRT1 in the germinal center of the follicles while the mantle zone shows nearly no staining to few positive cells. The classical HL-affected lymph nodes show a strong positivity of the diagnostic Hodgkin Reed-Sternberg cells. Notably, both the HL-derived cell lines and the Hodgkin Reed-Sternberg cells of the affected lymph nodes derive from germinal center-derived B cells. The study of SIRT1 distribution and expression on a larger number of biopsies might disclose a novel role for this histone/protein deacetylase as therapeutic target. © 2012 Wiley Periodicals, Inc. |
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Authors:
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Raffaele Frazzi; Riccardo Valli; Ione Tamagnini; Bruno Casali; Norbert Latruffe; Francesco Merli |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-26 |
Journal Detail:
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Title: International journal of cancer. Journal international du cancer Volume: - ISSN: 1097-0215 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0042124 Medline TA: Int J Cancer Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 UICC. |
Affiliation:
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Hematology Unit, Oncology Department, IRCCS - Arcispedale S.Maria Nuova, Reggio Emilia, Italy; Laboratory of Molecular Biology, Oncology Department, IRCCS - Arcispedale S.Maria Nuova, Reggio Emilia, Italy. raffaele.frazzi@asmn.re.it. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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