| Resveratrol enhances anti-proliferative effect of VACM-1/cul5 in T47D cancer cells. | |
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MedLine Citation:
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PMID: 20949323 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Vasopressin-activated calcium-mobilizing (VACM-1) protein is a cul-5 gene product that forms complexes with a subclass of ubiquitin E3 ligases involved in proteasomal protein degradation. The expression of VACM-1 cDNA in the T47D breast cancer cell line inhibits growth and decreases phosphorylation of mitogen activated protein kinase. Factors that regulate expression or stability of VACM-1 protein have not been identified, however. In our search to identify drugs/substances that may control VACM-1 protein expression, we examined the effects of resveratrol (trans-3,5,4'-trihydroxystilbene), a natural component in the human diet which inhibits tumor initiation and promotion. CMV vector and VACM-1 cDNA stably transfected T47D breast cancer-derived cells were treated with resveratrol and cell growth and VACM-1 protein concentrations were measured. Since the cellular mechanism of resveratrol-dependent inhibition of cell growth also involves the regulation of estrogen receptors, the effect of 17-β-estradiol and resveratrol on ERα levels and on cell growth was examined in control and in VACM-1 cDNA transfected cells. Our results demonstrate that antiproliferative effect of resveratrol observed in the control T47D cancer cells was significantly enhanced in VACM-1 cDNA transfected T47D cells. Western blot results indicated that resveratrol increased VACM-1 protein concentration. Finally, treatment with resveratrol for 24 and 48 h attenuated 17-β-estradiol induced increase in cell growth both in control and in VACM-1 cDNA transfected cells. The effect was significantly higher in the VACM-1 cDNA transfected cells when compared to controls. These results indicate that the antiproliferative effect of resveratrol may involve induction of VACM-1/cul5. |
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Authors:
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Justin Lubbers; Steven Lewis; Emily Harper; Michael P Hledin; Gabriel A Marquez; Alyssa E Johnson; Danelle R Graves; Maria A Burnatowska-Hledin |
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Publication Detail:
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Type: Journal Article Date: 2010-10-15 |
Journal Detail:
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Title: Cell biology and toxicology Volume: 27 ISSN: 1573-6822 ISO Abbreviation: Cell Biol. Toxicol. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8506639 Medline TA: Cell Biol Toxicol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 95-105 Citation Subset: IM |
Affiliation:
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Departments of Biology and Chemistry, Hope College, Holland, MI, 49422-9000, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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