Document Detail

Resveratrol attenuates L-DOPA-induced hydrogen peroxide toxicity in neuronal cells.
MedLine Citation:
PMID:  23114623     Owner:  NLM     Status:  MEDLINE    
A variety of polyphenol antioxidant compounds derived from natural products have demonstrated neuroprotective activity against neuronal cell death. The objective of this study was to investigate the effect of resveratrol (RESV) and bioflavonoids in attenuating hydrogen peroxide (H(2)O(2))-induced oxidative stress in neuronal cells. H2O2 levels were increased by the addition of L-3,4-dihydroxyphenylalanine (L-DOPA) to cultured dopaminergic SKNSH cells. H(2)O(2) was monitored by peroxyfluor-1, a selective H(2)O(2) optical probe. To examine the neuroprotective effects of RESV and bioflavonoids against L-DOPA, we cotreated RESV, quercetin, or (-) epigallocatechin gallate with L-DOPA and monitored for H(2)O(2) levels. The combination of RESV and L-DOPA was 50% more effective at reducing H(2)O(2) levels than the combination of quercetin or epigallocatechin gallate with L-DOPA. However, the combination of each antioxidant with L-DOPA was effective at preserving cell viability.
Carina S Peritore; Angela Ho; Bryan K Yamamoto; Scott E Schaus
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Neuroreport     Volume:  23     ISSN:  1473-558X     ISO Abbreviation:  Neuroreport     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-01     Completed Date:  2013-04-17     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  England    
Other Details:
Languages:  eng     Pagination:  989-94     Citation Subset:  IM    
Department of aChemistry bBiology, Boston University, Boston, Massachusetts 02215, USA.
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MeSH Terms
Antioxidants / pharmacology*
Catechin / analogs & derivatives,  pharmacology
Cell Line, Tumor / drug effects
Cell Survival / drug effects
Drug Evaluation, Preclinical
Drug Interactions
Fibroblasts / drug effects
Fluoresceins / analysis
Hydrogen Peroxide / toxicity*
Levodopa / antagonists & inhibitors*,  toxicity
Monoamine Oxidase / analysis
Nerve Tissue Proteins / analysis
Neuroblastoma / pathology
Neurons / drug effects*,  enzymology
Neuroprotective Agents / pharmacology*
Oxidative Stress / drug effects*
Quercetin / pharmacology
Stilbenes / pharmacology*
Tyrosine / pharmacology
Tyrosine 3-Monooxygenase / analysis
Grant Support
Reg. No./Substance:
0/Antioxidants; 0/Fluoresceins; 0/Levodopa; 0/Nerve Tissue Proteins; 0/Neuroprotective Agents; 0/Stilbenes; 0/peroxyfluor-1; 117-39-5/Quercetin; 154-23-4/Catechin; 55520-40-6/Tyrosine; 7722-84-1/Hydrogen Peroxide; BQM438CTEL/epigallocatechin gallate; EC 3-Monooxygenase; EC Oxidase; Q369O8926L/resveratrol

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