Document Detail


Resveratrol protects against oxidized LDL-induced breakage of the blood-brain barrier by lessening disruption of tight junctions and apoptotic insults to mouse cerebrovascular endothelial cells.
MedLine Citation:
PMID:  20980646     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cerebrovascular endothelial cells (CEC) comprise the blood-brain barrier (BBB). In a previous study, we showed that oxidized LDL (oxLDL) can induce apoptosis of mouse CEC. Resveratrol possesses chemopreventive potential. This study aimed to evaluate the effects of resveratrol on oxLDL-induced insults to mouse CEC and its possible mechanisms. Exposure of mouse CEC to 200 μmol/L oxLDL for 1 h did not cause cell death but significantly altered the permeability and transendothelial electrical resistance of the cell monolayer. However, resveratrol completely normalized such injury. As for the mechanisms, resveratrol completely protected oxLDL-induced disruption of F-actin and microtubule cytoskeletons as well as occludin and zona occludens-1 (ZO-1) tight junctions. The oxLDL-induced decreases in the mitochondrial membrane potential and intracellular ATP levels were normalized by resveratrol. Exposure of mouse CEC to 200 μmol/L oxLDL for 24 h elevated oxidative stress and simultaneously induced cell apoptosis. However, resveratrol partially protected against oxLDL-induced CEC apoptosis. The oxLDL-induced alterations in levels of Bcl-2, Bax, and cytochrome c were completely normalized by resveratrol. Consequently, resveratrol partially decreased oxLDL-induced activation of caspases-9 and -3. Therefore, in this study, we show that resveratrol can protect against oxLDL-induced damage of the BBB through protecting disruption of the tight junction structure and apoptotic insults to CEC.
Authors:
Yi-Ling Lin; Huai-Chia Chang; Ta-Liang Chen; Jin-Han Chang; Wen-Ta Chiu; Jia-Wei Lin; Ruei-Ming Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-27
Journal Detail:
Title:  The Journal of nutrition     Volume:  140     ISSN:  1541-6100     ISO Abbreviation:  J. Nutr.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-24     Completed Date:  2010-12-30     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2187-92     Citation Subset:  IM    
Affiliation:
Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Wan Fang Hospital, Taipei, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*
Blood-Brain Barrier*
Brain / blood supply*
Endothelium, Vascular / cytology,  drug effects*
Lipoproteins, LDL / pharmacology*
Mice
Stilbenes / pharmacology*
Tight Junctions / drug effects*
Chemical
Reg. No./Substance:
0/Lipoproteins, LDL; 0/Stilbenes; 0/oxidized low density lipoprotein; Q369O8926L/resveratrol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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