Document Detail

Resveratrol increases iodide trapping in the rat thyroid cell line FRTL-5.
MedLine Citation:
PMID:  20151827     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Resveratrol, a polyphenol found in grapes, exhibits several beneficial health effects by its antioxidant, antiinflammatory, and chemopreventive properties. The aim of the present study was to determine the effect of resveratrol on iodide trapping and efflux as well as its mode of action using FRTL-5 cells, having in mind the pivotal role of the natrium iodide symporter (NIS) in the treatment of differentiated thyroid cancers.
METHODS: Cells were treated with resveratrol for various times and doses, in the presence or absence of thyrotropin (TSH). Iodide trapping, iodide efflux, rat NIS (rNIS) protein expression, and cyclic AMP (cAMP) production were evaluated.
RESULTS: Resveratrol increased iodide trapping in a time-dependent (optimal 6 hours) and dose-dependent (100 microM) way in the presence of TSH. It showed an additive effect when concomitantly added with an optimal dose of TSH. Resveratrol (50 microM) increased (threefold) rNIS protein expression. In TSH-deprived cells, resveratrol also provoked an increase in rNIS protein (>3-fold in 6 hours) with an optimum at 40 microM. Resveratrol did not inhibit iodide efflux from FRTL-5 cells. It neither increased intracellular cAMP nor induced the arborization of living cells, two TSH-induced effects. A non-cAMP mode of action is highly suspected.
CONCLUSIONS: Resveratrol increases iodide trapping in FRTL-5 cells, increasing iodide influx and rNIS protein level even in the absence of TSH. It has an additive effect with TSH. Consequently, resveratrol could be a promising molecule for radioiodide therapy in follicular and papillary differentiated thyroid carcinoma in association with recombinant human TSH.
Hichem Sebai; Sonia Hovsépian; Elodie Ristorcelli; Ezzedine Aouani; Dominique Lombardo; Guy Fayet
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Thyroid : official journal of the American Thyroid Association     Volume:  20     ISSN:  1557-9077     ISO Abbreviation:  Thyroid     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-15     Completed Date:  2010-04-29     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  9104317     Medline TA:  Thyroid     Country:  United States    
Other Details:
Languages:  eng     Pagination:  195-203     Citation Subset:  IM    
INSERM UMR-911 and Research Center in Biological and Oncopharmacological Oncology (CrO2), School of Medicine-Timone, Aix-Marseille University, France.
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MeSH Terms
Cell Line
Cyclic AMP / biosynthesis
Dose-Response Relationship, Drug
Iodides / metabolism*
Stilbenes / administration & dosage,  pharmacology*
Symporters / biosynthesis*
Thyroid Gland / drug effects,  metabolism*
Thyrotropin / pharmacology
Reg. No./Substance:
0/Iodides; 0/Stilbenes; 0/Symporters; 0/sodium-iodide symporter; 60-92-4/Cyclic AMP; 9002-71-5/Thyrotropin; Q369O8926L/resveratrol
Comment In:
Thyroid. 2010 Feb;20(2):125-6   [PMID:  20151819 ]

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