| Resuscitation affects microcirculatory polymorphonuclear leukocyte behavior after hemorrhagic shock: role of hypertonic saline and pentoxifylline. | |
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MedLine Citation:
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PMID: 15229363 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously shown that lung injury following fluid resuscitation either with hypertonic saline (HS) or lactated Ringer's (LR) plus pentoxifylline (PTX) attenuated acute lung injury when compared with LR resuscitation. The objective of the present study is to determine whether our previous observations are accompanied by changes in polymorphonu-clear leukocyte (PMN) behavior. To study this, PMN-endothelial cell interactions, microcirculatory blood flow, lung histology, lung PMN infiltration (MPO, Myeloperoxidase), and lung intra-cellular adhesion molecule-1 (ICAM-1) expression were assessed in a controlled hemorrhagic shock model followed by LR, HS, and LR+PTX resuscitation in rodents. Rats (240-300 g) were bled to a mean arterial pressure (MAP) of 35 mm Hg for 1 hr and then randomized into three groups: HS (7.5% NaCl, 4 ml/kg); LR (3x shed blood); and LR+PTX (25 mg/kg). Additionally, total shed blood was reinfused. A sham group underwent no shock and no treatment. The internal spermatic fascia was exteriorized and the microcirculation was observed by closed-circuit TV coupled to a microscope, 2 and 6 hrs after treatment. The number of leukocytes sticking to the venular endothelium was determined 2 hrs after fluid resuscitation. Microcirculatory blood flow was measured by an optical Doppler velocimeter. Lung histology and lung MPO immunostaining were assessed at 6 hrs, and lung ICAM-1 expression was determined by immunostaining at 2 hrs following fluid resuscitation. Two hours after treatment, HS (1.4 +/- 0.4), LR+PTX (1.7 +/- 0.3), and sham (0.4 +/- 0.2) groups presented significant reductions in leukocyte adherence (cells/100 microm venule length), compared with the LR group (4.0 +/- 0.9, P < 0.05). No differences were observed 6 hrs after treatment on leukocyte adherence and microcirculatory blood flow. ICAM-1 expression was significantly higher in LR-treated animals compared with the HS, LR+PTX, and sham groups (P < 0.01). PMN infiltration and overall lung injury were significantly attenuated by HS and LR+PTX. These results support earlier studies that indicated the potential application of HS and PTX in shock therapy and the increase in PMN-endothelial cell interaction and lung injury after LR resuscitation. |
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Authors:
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Margareth M Yada-Langui; Edna A Anjos-Valotta; Paulina Sannomiya; Mauricio Rocha e Silva; Raul Coimbra |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental biology and medicine (Maywood, N.J.) Volume: 229 ISSN: 1535-3702 ISO Abbreviation: Exp. Biol. Med. (Maywood) Publication Date: 2004 Jul |
Date Detail:
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Created Date: 2004-07-01 Completed Date: 2004-08-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100973463 Medline TA: Exp Biol Med (Maywood) Country: United States |
Other Details:
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Languages: eng Pagination: 684-93 Citation Subset: IM |
Affiliation:
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Division of Trauma, 8896, University of California San Diego School of Medicine, 200 West Arbor Drive, San Diego, CA 92103-8896, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Disease Models, Animal Male Microcirculation / drug effects, physiopathology* Neutrophils / drug effects, physiology* Pentoxifylline / pharmacology* Rats Rats, Wistar Saline Solution, Hypertonic / pharmacology* Shock, Hemorrhagic / blood* |
| Chemical | |
Reg. No./Substance:
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0/Saline Solution, Hypertonic; 6493-05-6/Pentoxifylline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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