| Resuscitation of acid-injured Salmonella in enrichment broth, in apple juice and on the surfaces of fresh-cut cucumber and apple. | |
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MedLine Citation:
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PMID: 16305675 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: To investigate the resuscitation of acid-injured Salmonella enterica in selected enrichment broths, in apple juice and on cut surfaces of apple and cucumber slices. METHODS AND RESULTS: Following exposure to 2.4% acetic acid for 7 min, S. enterica (serovars Mbandaka, Chester and Newport) cells were used to inoculate enrichment broths, phosphate-buffered saline (PBS), apple juice and fruit slices. Injured Salmonella cells resuscitated and regained the ability to form colonies on selective agar (Xylose-Lysine-Tergitol 4) if they were incubated in lactose broth (LB), universal pre-enrichment broth (UPB) or buffered peptone water (BPW), but not in tetrathionate broth, PBS or apple juice. The resuscitation occurred at a significantly (P > 0.05) faster rate in UPB than in LB or BPW. The resuscitation also occurred on the surfaces of fresh-cut cucumber at 20 degrees C, but not at 4 degrees C. CONCLUSIONS: Acid-injured Salmonella cells resuscitated in nonselective enrichment broths at different rates, but not in selective enrichment broth, apple juice, PBS or on fresh-cut apple. SIGNIFICANCE AND IMPACT OF THE STUDY: Pre-enrichment of food samples in UPB prior to selective enrichment is recommended. Injured Salmonella cells have the ability to resuscitate on fresh-cut surfaces of cucumber when stored at abusive temperatures. |
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Authors:
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C-H Liao; W F Fett |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Letters in applied microbiology Volume: 41 ISSN: 0266-8254 ISO Abbreviation: Lett. Appl. Microbiol. Publication Date: 2005 |
Date Detail:
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Created Date: 2005-11-24 Completed Date: 2006-02-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8510094 Medline TA: Lett Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 487-92 Citation Subset: IM |
Affiliation:
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Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, Wyndmoor, PA 19038, USA. cliao@errc.ars.usda.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acids
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pharmacology Cucumis sativus / chemistry*, microbiology Culture Media Food Microbiology Malus / chemistry*, microbiology Salmonella / drug effects, genetics, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Acids; 0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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